Evidence map›Paper›PMID 40834152›Full record

ArticleEinstein (Sao Paulo, Brazil)2025

High levels of a comprehensive set of matrix metalloproteinases in endometriotic lesions: validating the key role of cellular senescence in endometriosis pathogenesis.

Laura Palmieri, Helena Malvezzi, Bruna Cestari de Azevedo, Eduardo Varejão Díaz Placencia, Eliane Antonioli, Sérgio Podgaec

Abstract read
In one paragraph

Article in Einstein (Sao Paulo, Brazil), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Laura PalmieriAcademic, Faculdade Israelita de Ciências da Saúde Albert Einstein, Hospital Israelita Albert Einstein, São Paulo, SP, Brazil.ORCID http://orcid.org/0000-0002-2998-8679
Helena MalvezziResearcher, Instituto Israelita de Ensino e Pesquisa Albert Einstein, Hospital Israelita Albert Einstein, São Paulo, SP, Brazil.ORCID http://orcid.org/0000-0002-5504-6116
Bruna Cestari de AzevedoHospital Israelita Albert Einstein, São Paulo, SP, Brazil.ORCID http://orcid.org/0000-0002-3495-0798
Eduardo Varejão Díaz PlacenciaPostgraduate Program, Faculdade Israelita de Ciências da Saúde Albert Einstein, Hospital Israelita Albert Einstein, São Paulo, SP, Brazil.ORCID http://orcid.org/0000-0001-8066-7224
Eliane AntonioliHospital Israelita Albert Einstein, São Paulo, SP, Brazil.ORCID http://orcid.org/0000-0002-8563-877X
Sérgio PodgaecHospital Israelita Albert Einstein, São Paulo, SP, Brazil.ORCID http://orcid.org/0000-0002-9760-6003

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundThis study reveals elevated levels of MMP-1, MMP-2, and MMP-3 in endometriotic lesions, potentially reflecting a senescenceassociated secretory phenotype. No corresponding increase was observed in peritoneal fluid, possibly due to technical or sample-related limitations. These findings support the potential of MMPs as biomarkers and therapeutic targets in endometriosis.

backgroundMMP-1, MMP-2, and MMP-3 are upregulated in endometriotic lesions.

backgroundThe study findings suggest a senescence-associted secretory phenotype in endometriosis.

backgroundThe study findings support targeting senescence pathways in endometriosis.

objectiveTo investigate the roles of cellular senescence and dysfunctional extracellular matrix metalloproteinases (MMPs) in perpetuating chronic inflammation and facilitating the establishment of endometriotic lesions. By analyzing the MMP activity in the endometrial tissue and peritoneal fluid, we aimed to obtain novel insights into the molecular mechanisms underlying endometriosis-related pathophysiology.

methodsThe endometrial tissue and peritoneal fluid samples were collected laparoscopically from 12 women with endometriosis and 16 healthy controls. Gelatin zymography was performed to assess the activity of MMP-2, and multiplex assays were performed to determine the concentrations of MMP-1 and MMP-3 proteins. Statistical analyses were performed using Generalized Linear Models (GzLM) and SPSS software.

resultsGelatin zymography revealed higher pro-MMP-2 activity in endometriotic lesions than in eutopic and control endometrium. However, no differences were observed in peritoneal fluid samples. Additionally, MMP-1 and MMP-3 protein levels were elevated in endometriotic lesions compared with those in the eutopic endometrium, whereas only MMP-3 was increased compared with that in the control. No statistical significance was observed for MMP-2, MMP-1, and MMP-3 in the peritoneal fluid samples.

conclusionIncreased levels of MMP-1, MMP-2, and MMP-3 in endometriotic lesions indicate that endometriosis may have a unique metabolomic signature linked to cell cycle arrest and inflammation. This may contribute to the pathogenesis of endometriosis by facilitating implantation of ectopic endometrium-like tissue in a disturbed immune environment.

Indexed as

Cellular SenescenceEndometriosisMatrix MetalloproteinasesAdultAscitic FluidBiomarkersCase-Control StudiesEndometriumFemaleHumansMatrix Metalloproteinase 1Matrix Metalloproteinase 2Matrix Metalloproteinase 3Young AdultBiomarkersMatrix Metalloproteinase 1Matrix Metalloproteinase 2Matrix Metalloproteinase 3Matrix Metalloproteinases

Identifiers

PMID40834152
PMCPMC12539843

What Socratic holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.