Evidence map›Paper›PMID 40838554›Full record

ArticleAngewandte Chemie (International ed. in English)2025

Green Fluorescent Protein SELEX: Immobilization Chemistry and His-Tag Epitope Bias.

Stefen Stangherlin, Tyler Malloch, Anthony J Clarke, Juewen Liu

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Angewandte Chemie (International ed. in English), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Green Fluorescent Protein SELEX: Immobilization Chemistry and His-Tag Epitope Bias.Angewandte Chemie (International ed. in English) · 2025
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Stefen StangherlinDepartment of Chemistry, Waterloo Institute for Nanotechnology, University of Waterloo, 200 University Avenue West, Waterloo, Ontario, N2L 3G1, Canada.
Tyler MallochDepartment of Molecular and Cellular Biology, University of Guelph, 50 Stone Road East, Guelph, Ontario, N1G 2W1, Canada.
Anthony J ClarkeDepartment of Molecular and Cellular Biology, University of Guelph, 50 Stone Road East, Guelph, Ontario, N1G 2W1, Canada.
Juewen LiuDepartment of Chemistry, Waterloo Institute for Nanotechnology, University of Waterloo, 200 University Avenue West, Waterloo, Ontario, N2L 3G1, Canada.ORCID 0000-0001-5918-9336

Funding

(CFI) Major Science Initiatives (MSI) 42687Natural Sciences and Engineering Research Council of Canada
6 · The paper itself

Abstract

Despite numerous DNA aptamers for proteins having been reported, a model system allowing the use of cost-effective proteins, unmodified DNA, and convenient homogeneous assays is still lacking, which has in turn limited not only fundamental studies of aptamers but also their translation to practical applications. Herein, three separate green fluorescent protein (GFP) selections were carried out using both non-tagged and His-tagged GFP immobilized on either NHS-activated resin or Co

Indexed as

Aptamers, NucleotideEpitopesGreen Fluorescent ProteinsHistidineSELEX Aptamer TechniqueAptamers, NucleotideEpitopesGreen Fluorescent ProteinsHistidineAptamersDNAFluorescence polarizationProteinsSELEX

Identifiers

PMID40838554
PMCPMC12462738

What Socratic holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.