Evidence mapPaperPMID 40839021Full record

ArticleRice (New York, N.Y.)2025

Alternative polyadenylation and metabolic profiling in young panicle development of hybrid rice and its parents.

Guangliang Wu, Shangwei Yu, Junru Fu, Linjuan Ouyang, Lifang Hu, Jianmin Bian, Xiaorong Chen, Jie Xu, Dahu Zhou, Haohua He and 1 more

Erratum issuedAbstract read
In one paragraph

Article in Rice (New York, N.Y.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
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4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

11 authors.

Guangliang WuKey Laboratory of Crop Physiology, Ecology and Genetic Breeding, Ministry of Education, Jiangxi Agricultural University, Nanchang, 330045, China.
Shangwei YuKey Laboratory of Crop Physiology, Ecology and Genetic Breeding, Ministry of Education, Jiangxi Agricultural University, Nanchang, 330045, China.
Junru FuKey Laboratory of Crop Physiology, Ecology and Genetic Breeding, Ministry of Education, Jiangxi Agricultural University, Nanchang, 330045, China.
Linjuan OuyangKey Laboratory of Crop Physiology, Ecology and Genetic Breeding, Ministry of Education, Jiangxi Agricultural University, Nanchang, 330045, China.
Lifang HuKey Laboratory of Crop Physiology, Ecology and Genetic Breeding, Ministry of Education, Jiangxi Agricultural University, Nanchang, 330045, China.
Jianmin BianKey Laboratory of Crop Physiology, Ecology and Genetic Breeding, Ministry of Education, Jiangxi Agricultural University, Nanchang, 330045, China.
Xiaorong ChenKey Laboratory of Crop Physiology, Ecology and Genetic Breeding, Ministry of Education, Jiangxi Agricultural University, Nanchang, 330045, China.
Jie XuKey Laboratory of Crop Physiology, Ecology and Genetic Breeding, Ministry of Education, Jiangxi Agricultural University, Nanchang, 330045, China.
Dahu ZhouKey Laboratory of Crop Physiology, Ecology and Genetic Breeding, Ministry of Education, Jiangxi Agricultural University, Nanchang, 330045, China.
Haohua HeKey Laboratory of Crop Physiology, Ecology and Genetic Breeding, Ministry of Education, Jiangxi Agricultural University, Nanchang, 330045, China. hhhua64@163.com.
Haihui FuKey Laboratory of Crop Physiology, Ecology and Genetic Breeding, Ministry of Education, Jiangxi Agricultural University, Nanchang, 330045, China. fhh819@163.com.

Funding

National Natural Science Foundation of China 32060045National Natural Science Foundation of China,China 32360650the Double Thousand Plan of Jiangxi Province jxsq2019101057
6 · The paper itself

Abstract

Alternative polyadenylation (APA) is a widespread post-transcriptional regulatory mechanism in eukaryotes that modulates gene expression by generating transcript variants. The development of young panicles in rice is a critical stage that determines grain number and weight. However, the regulatory mechanisms and inheritance patterns of APA during this process remain poorly understood. In this study, full-length isoform sequencing (Iso-seq) and metabolome were employed to investigate APA dynamics in the young panicle of hybrid rice variety Wufengyou T025 and in parent lines, Wufeng B and Changhui T025. This analysis revealed that approximately 80% of genes possessed two or more polyadenylation (pA) sites. These APA genes were predominantly enriched in the pathways associated with rice spikelet development, including response to external photoperiod changes, energy production and transportation, protein signal exchange, and amino acid metabolism. Notably, transcripts with a shortened 3'-untranslated region (3'UTR) exhibited elevated expression levels of their corresponding genes, suggesting that APA plays an important role in modulating gene expression. Furthermore, the variable 3'UTR of the 25% differentially expressed APA genes contained numerous miRNA binding sites, including osa-miR1848 and osa-miR5075, which are known to influence spikelet development. In the offspring, the expression levels of core APA factors during young panicle development were generally downregulated compared to the parental lines. Additionally, metabolomic analysis identified 209 and 164 differentially abundant metabolites in the offspring relative to Wufeng B and Changhui T025, respectively. Intriguingly, some of the enriched metabolic pathways overlapped with those of differentially expressed APA genes, implying that APA may influence small-molecule metabolites in pathways related to spike development. Collectively, these findings are valuable for understanding the regulation of APA and its genetic basis in young panicle development, offering new insights into the molecular mechanisms underlying this critical development stage.

Indexed as

Alternative polyadenylationHybrid riceIso-seqMetabolomePanicles development

Identifiers

PMID40839021
PMCPMC12370618

What Socratic holds

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.