Evidence map›Paper›PMID 40839321›Full record

ArticleGenes & genomics2025

The role of RANBP1 in regulating MiRNA expression and apoptosis in breast cancer cells.

Pengxia Song, Lin Zhang, Huiping Qiu, Xingchen Zhou, Yanhui Cao, Jie Liu, Xin Zheng, Yubin Liu, Shuihong Yao

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PubMed Full text at PMC Publisher
In one paragraph

Article in Genes & genomics, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Pengxia Song *Medical School, Quzhou College of Technology, No.18, Jiangyuan Road, Quzhou, 324000, Zhejiang Province, China.
Lin Zhang *Quzhou People's Hospital, The Quzhou Affiliated Hospital of Wenzhou Medical University, Quzhou, 324000, China.
Huiping QiuMedical School, Quzhou College of Technology, No.18, Jiangyuan Road, Quzhou, 324000, Zhejiang Province, China.
Xingchen ZhouDivision of (Bio) Pharmaceutics, Institute of Zhejiang University-Quzhou, 99 Zheda Road, Quzhou, 324000, Zhejiang Province, China.
Yanhui CaoMedical School, Quzhou College of Technology, No.18, Jiangyuan Road, Quzhou, 324000, Zhejiang Province, China.
Jie LiuMedical School, Quzhou College of Technology, No.18, Jiangyuan Road, Quzhou, 324000, Zhejiang Province, China.
Xin ZhengMedical School, Quzhou College of Technology, No.18, Jiangyuan Road, Quzhou, 324000, Zhejiang Province, China.
Yubin LiuDivision of (Bio) Pharmaceutics, Institute of Zhejiang University-Quzhou, 99 Zheda Road, Quzhou, 324000, Zhejiang Province, China. lyb091@zju.edu.cn.
Shuihong YaoMedical School, Quzhou College of Technology, No.18, Jiangyuan Road, Quzhou, 324000, Zhejiang Province, China. qzxyysh@126.com.ORCID http://orcid.org/0000-0001-8963-2263

Funding

the science and technology planning project of Quzhou 2022K110the science and technology planning project of Quzhou 2024ZD090
6 · The paper itself

Abstract

backgroundBreast cancer poses a huge health threat in China. Emerging evidence has indicated that RANBP1 is associated with poor prognosis of breast cancer and has been shown to influence miRNA expression in colorectal cancer. But its role in breast cancer remains unclear.

objectiveThe purpose of this research is to construct the sh-RANBP1 cell line derived from a human breast cancer cell line in order to investigate the impact of low RANBP1 expression on the expression network of breast cancer-associated miRNAs and mRNAs.

methodsWe constructed the sh-RANBP1 cell line from the human breast cancer cell line MDA-MB-231 and analyzed the significant differential expression between the two groups using miRNA sequencing and mRNA sequencing of both the sh-RANBP1 group and the control group. The differential miRNA-mRNA regulatory network for the two groups was established by intersecting target predictions with the differential expression data. The results were further verified using Western blotting (WB), quantitative PCR (qRT-PCR), and flow cytometry.

resultsIn this study, we compared the expression levels of miRNA and mRNA between the sh-RANBP1 group and the control group. Our findings indicate that sh-RANBP1 influences miRNA expression, revealing significant differences based on the interaction network of miRNA and mRNA. The genes involved are associated with pathways such as apoptosis. Subsequent WB and qRT-PCR results further validated our findings. Finally, flow cytometry confirmed an increased proportion of apoptosis in the sh-RANBP1 interference group.

conclusionIn summary, sh-RANBP1 affects miRNA expression in breast cancer cells, then regulates the expression of mRNA, and ultimately increases the proportion of apoptosis in breast cancer cells.

Indexed as

ApoptosisBreast NeoplasmsMicroRNAsNuclear ProteinsCell Line, TumorFemaleGene Expression Regulation, NeoplasticGene Regulatory NetworksHumansRNA, MessengerMicroRNAsNuclear Proteinsran-binding protein 1RNA, MessengerApoptosisBreast cancermiRNAmRNAsh-RANBP1

Identifiers

PMID40839321
PMCPMC12515217

What Socratic holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.