Evidence map›Paper›PMID 40847241›Full record

ReviewNature protocols2026

Metabolomics using anion-exchange chromatography mass spectrometry for the analysis of cells, tissues and biofluids.

Rachel Williams, John Walsby-Tickle, Ingvild Comfort Hvinden, Isabelle Legge, Tereza Kacerova, KyoungEun Vicky Lee, Mariya Misheva, David Hauton, Judith B Ngere, John D Sidda and 3 more

Abstract readReview
PubMed Publisher
In one paragraph

Review in Nature protocols, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Review
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Rachel WilliamsDepartment of Chemistry, University of Oxford, Oxford, UK.ORCID 0000-0001-9069-6416
John Walsby-TickleDepartment of Chemistry, University of Oxford, Oxford, UK.ORCID 0000-0002-1287-9580
Ingvild Comfort HvindenDepartment of Chemistry, University of Oxford, Oxford, UK.
Isabelle LeggeDepartment of Chemistry, University of Oxford, Oxford, UK.ORCID 0000-0001-5913-4335
Tereza KacerovaDepartment of Chemistry, University of Oxford, Oxford, UK.ORCID 0000-0002-9477-0182
KyoungEun Vicky LeeDepartment of Chemistry, University of Oxford, Oxford, UK.
Mariya MishevaDepartment of Chemistry, University of Oxford, Oxford, UK.
David HautonDepartment of Chemistry, University of Oxford, Oxford, UK.
Judith B NgereDepartment of Chemistry, University of Oxford, Oxford, UK.
John D SiddaDepartment of Chemistry, University of Oxford, Oxford, UK.ORCID 0000-0002-3135-0885
Elisabete PiresDepartment of Chemistry, University of Oxford, Oxford, UK.ORCID 0000-0001-8646-062X
Tom Cadoux-HudsonDepartment of Chemistry, University of Oxford, Oxford, UK.
James S O McCullaghDepartment of Chemistry, University of Oxford, Oxford, UK. james.mccullagh@chem.ox.ac.uk.ORCID 0000-0003-4733-1205

Funding

Oxford University | John Fell Fund, University of Oxford (John Fell OUP Research Fund) JFF142/116RCUK | Biotechnology and Biological Sciences Research Council (BBSRC) BB/R013829/1RCUK | Engineering and Physical Sciences Research Council (EPSRC) EP/W524311/1Thermo Fisher Scientific (Thermo Fisher Scientific Inc.) DPhil fundingWellcome Trust (Wellcome) 204483/Z/16/Z
6 · The paper itself

Abstract

The direct coupling of ion-exchange chromatography with mass spectrometry using electrochemical ion suppression creates a hyphenated technique with selectivity and specificity for the analysis of highly polar and ionic compounds. The technique has enabled new applications in environmental chemistry, food chemistry, forensics, cell biology and, more recently, metabolomics. Robust, reproducible and quantitative methods for the analysis of highly polar and ionic metabolites help meet a longstanding analytical need in metabolomics. Here, we provide step-by-step instructions for both untargeted and semi-targeted metabolite analysis from cell, tissue or biofluid samples by using anion-exchange chromatography-high-resolution tandem mass spectrometry (AEC-MS/MS). The method requires minimal sample preparation and is robust, sensitive and selective. It provides comprehensive coverage of hundreds of metabolites found in primary and secondary metabolic pathways, including glycolysis, the pentose phosphate pathway, the tricarboxylic acid cycle, purine and pyrimidine metabolism, amino acid degradation and redox metabolism. An inline electrolytic ion suppressor is used to quantitatively neutralize OH

Indexed as

Body FluidsMetabolomicsTandem Mass SpectrometryAnimalsChromatography, Ion ExchangeHumans

Identifiers

PMID40847241

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.