Evidence map›Paper›PMID 40847747›Full record

ArticlePlant biotechnology journal2025

Dual-Layer Transcriptional-Protein Regulation by HvAP2-12 Represses HvAP2-18 Activity to Fine-Tune Barley Starch Synthesis.

Jinjin Ding, Yulong Li, Jing Liu, Na Lin, David Seung, Qiang Xu, Yazhou Zhang, Huaping Tang, Pengfei Qi, Mei Deng and 5 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Plant biotechnology journal, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Review
  5. Review
  6. Proteomic Analysis of Lotus-DerivedPlants (Basel, Switzerland) · 2026
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors.

Jinjin DingState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan, China.
Yulong LiState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan, China.
Jing LiuState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan, China.
Na LinFaculty of Agriculture, Forestry and Food Engineering, Yibin University, Yibin, Sichuan, China.
David SeungJohn Innes Centre, Norwich Research Park, Norwich, UK.ORCID https://orcid.org/0000-0003-3905-3647
Qiang XuState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan, China.
Yazhou ZhangState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan, China.
Huaping TangState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan, China.
Pengfei QiState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan, China.ORCID https://orcid.org/0000-0002-7772-9591
Mei DengState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan, China.
Jian MaState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan, China.ORCID https://orcid.org/0000-0001-6265-7911
Guoyue ChenState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan, China.
Jirui WangState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan, China.
Yuming WeiState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan, China.
Qiantao JiangState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan, China.ORCID https://orcid.org/0000-0003-3192-3732

Funding

Open Foundation of Key Laboratory of Wuliangye-flavor Liquor Solid-state Fermentation 2023JJ011Sichuan Science and Technology Program 2024YFNH0023Sichuan Science and Technology Program 2025YFHZ0113Yibin University Research Projects 2022YY06
6 · The paper itself

Abstract

Starch content and composition are key factors determining yield and quality of barley, but the molecular mechanisms regulating barley starch synthesis remain unclear. In this study, we identified an APETALA2/Ethylene-Responsive factor (AP2/ERF) family gene HvAP2-12, which has typical transcription factor characteristics. Overexpression of HvAP2-12 had no effect in total starch and amylose content, whereas the gene-edited mutant lines showed increased total starch and significantly decreased amylose content. Further analysis revealed that HvAP2-12 does not directly bind to the promoter of major starch synthesis genes. However, it binds to another AP2/ERF family member, the HvAP2-18 promoter and interacts with the HvAP2-18 protein. HvAP2-18 also affects starch synthesis in barley grains, and its overexpression increases total starch content while significantly reducing amylose content. HvAP2-18 binds directly to the promoters of key starch synthesis genes HvAGP-S1 and HvSBE1, activating their expression. Additionally, HvAP2-12 inhibits HvAP2-18 from binding to HvAGP-S1 and HvSBE1 promoters. Based on these findings, we propose that HvAP2-12 and HvAP2-18 play critical roles in the regulation of starch genes and in determining barley grain quality.

Indexed as

HordeumPlant ProteinsStarchTranscription FactorsAmyloseGene Expression Regulation, PlantPlants, Genetically ModifiedPromoter Regions, GeneticAmylosePlant ProteinsStarchTranscription FactorsbarleyHvAP2‐12HvAP2‐18starch synthesistranscription factors

Identifiers

PMID40847747
PMCPMC12665055

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.