Evidence map›Paper›PMID 40851369›Full record

ArticleProtein & cell2025

Specific pathogen free ten gene-edited donor pigs for xenotransplantation.

Kaixiang Xu, Heng Zhao, Baoyu Jia, Jiaoxiang Wang, Nazar Ali Mohammed Ali Siddig, Muhammad Ameen Jamal, Aqiang Mao, Kai Liu, Wenjie Cheng, Chang Yang and 16 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Protein & cell, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed.

  1. Review
  2. Article
  3. Review
  4. Generation of tetraploid organs in mice.Zoological research · 2026
    Article
  5. Review
  6. Article
  7. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

26 authors.

Kaixiang XuYunnan Province Key Laboratory for Porcine Gene Editing and Xenotransplantation, Yunnan Agricultural University, Kunming 650201, China.ORCID 0000-0001-6665-3360
Heng ZhaoYunnan Province Key Laboratory for Porcine Gene Editing and Xenotransplantation, Yunnan Agricultural University, Kunming 650201, China.ORCID 0000-0002-9320-5894
Baoyu JiaYunnan Province Key Laboratory for Porcine Gene Editing and Xenotransplantation, Yunnan Agricultural University, Kunming 650201, China.
Jiaoxiang WangYunnan Province Key Laboratory for Porcine Gene Editing and Xenotransplantation, Yunnan Agricultural University, Kunming 650201, China.
Nazar Ali Mohammed Ali SiddigYunnan Province Key Laboratory for Porcine Gene Editing and Xenotransplantation, Yunnan Agricultural University, Kunming 650201, China.
Muhammad Ameen JamalYunnan Province Key Laboratory for Porcine Gene Editing and Xenotransplantation, Yunnan Agricultural University, Kunming 650201, China.ORCID 0000-0002-8364-6389
Aqiang MaoYunnan Province Key Laboratory for Porcine Gene Editing and Xenotransplantation, Yunnan Agricultural University, Kunming 650201, China.
Kai LiuYunnan Province Key Laboratory for Porcine Gene Editing and Xenotransplantation, Yunnan Agricultural University, Kunming 650201, China.
Wenjie ChengYunnan Province Key Laboratory for Porcine Gene Editing and Xenotransplantation, Yunnan Agricultural University, Kunming 650201, China.
Chang YangYunnan Province Key Laboratory for Porcine Gene Editing and Xenotransplantation, Yunnan Agricultural University, Kunming 650201, China.
Taiyun WeiYunnan Province Key Laboratory for Porcine Gene Editing and Xenotransplantation, Yunnan Agricultural University, Kunming 650201, China.
Feiyan ZhuYunnan Province Key Laboratory for Porcine Gene Editing and Xenotransplantation, Yunnan Agricultural University, Kunming 650201, China.
Xiaoyin HuoYunnan Province Key Laboratory for Porcine Gene Editing and Xenotransplantation, Yunnan Agricultural University, Kunming 650201, China.
Deling JiaoYunnan Province Key Laboratory for Porcine Gene Editing and Xenotransplantation, Yunnan Agricultural University, Kunming 650201, China.ORCID 0000-0003-3894-7665
Jianxiong GuoYunnan Province Key Laboratory for Porcine Gene Editing and Xenotransplantation, Yunnan Agricultural University, Kunming 650201, China.
Hongfang ZhaoYunnan Province Key Laboratory for Porcine Gene Editing and Xenotransplantation, Yunnan Agricultural University, Kunming 650201, China.
Wenmin ChengYunnan Province Key Laboratory for Porcine Gene Editing and Xenotransplantation, Yunnan Agricultural University, Kunming 650201, China.
Yuemiao ZhangRenal Division, Key Laboratory of Renal Disease, Peking University First Hospital, Peking University Institute of Nephrology, Beijing 100034, China.
Xiangyu ZhangRenal Division, Key Laboratory of Renal Disease, Peking University First Hospital, Peking University Institute of Nephrology, Beijing 100034, China.
Lei JiangRenal Division, Key Laboratory of Renal Disease, Peking University First Hospital, Peking University Institute of Nephrology, Beijing 100034, China.
Zijie ZhangBio-X Center for Interdisciplinary Innovation, Yunnan University, Kunming 650091, China.ORCID 0000-0002-4900-506X
Wei ZhangThe First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 311103, China.
Tingbo LiangThe First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 311103, China.ORCID 0000-0003-0143-3353
Hong-Ye ZhaoYunnan Province Key Laboratory for Porcine Gene Editing and Xenotransplantation, Yunnan Agricultural University, Kunming 650201, China.ORCID 0000-0002-5663-1093
Bei-Cheng SunThe First Affiliation Hospital of Anhui Medical University, Hefei 230022, China.
Hong-Jiang WeiYunnan Province Key Laboratory for Porcine Gene Editing and Xenotransplantation, Yunnan Agricultural University, Kunming 650201, China.ORCID 0000-0002-5900-3033

Funding

Basic Strengthening Program 2023-JCJQ-ZD-118-00/173Major Science and Technology Project of Yunnan Province 202102AA100054Major Science and Technology Project of Yunnan Province 202102AA310047Major Science and Technology Project, Yunnan Province 202102AA100054Major Science and Technology Project, Yunnan Province 202102AA310047National Key Research and Development Program of China 2019YFA0110700Xingdian Talent Support Program XDYC-KJLJ-2022-0004
6 · The paper itself

Abstract

Xenotransplantation has entered the clinical phase in an effort to address the global organ shortage. However, recent clinical studies have revealed that current xenografts from gene-edited (GE) pigs still pose a risk of immune rejection and biosafety concerns. In this study, we successfully produced a large batch of 582 GE cloned (GEC) pigs with 10-(GTKO/CMAHKO/β4GalNT2KO/hCD46/ hCD55/hCD59/hTBM/hCD39/hEPCR/hCD47) gene edits via gene editing and somatic cell cloning technologies, and successfully obtained the F1 generation. Phenotypic characterization of 10-GEC pigs revealed the deletion of three xenoantigens and the expression of seven human transgenes across various tissues. Digital droplet polymerase chain reaction and whole-genome sequencing revealed two copies of hCD46/hCD55/hCD59/hTBM/hCD39 and one copy of hEPCR/hCD47 in the pig genome with minimal off-target effects or damage to the porcine functional genes. The validation results showed that 10-GEC pigs could effectively inhibit attacks from human antibodies, complement and macrophages on porcine endothelial cells, and alleviated coagulation abnormalities between pigs and humans. Large-scale screening of pathogens revealed no evidence of 47 pathogens, including cytomegalovirus, in our 10-GEC pigs. Kidney, heart and liver xenografts from these 10-GEC pigs were transplanted into nonhuman primates (NHPs), which worked normally without hyperacute rejection (HAR). Among NHPs, the heart and liver orthotopic transplant recipients survived for 3 and 4 days, respectively, while the two kidney transplant recipients survived for 23 and 16 days, respectively. Pathological analysis showed interstitial hemorrhage and fibrosis, cellular hyperplasia with minor antibodies and complement deposition, but significantly reduced infiltration of CD68+ macrophages in 10-GEC pig kidney xenografts. In summary, we successfully produced specific pathogen-free 10-GEC donor pigs that resulted in effective mitigation of immune rejection upon multiorgan transplantation to NHPs.

Indexed as

Gene EditingTransplantation, HeterologousAnimalsAnimals, Genetically ModifiedGraft RejectionHumansSwinedonor piggene editingimmune rejectionpathogenic microorganismsxenotransplantation

Identifiers

PMID40851369
PMCPMC12742852

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.