ArticleChinese medicine2025
Multi-omics analysis reveals ACOT1 as the key target of piperine in Piper Longum-mediated gastric cancer treatment.
Article in Chinese medicine, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
What it found
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Who cites it
4 citing papers in PubMed.
- Acyl-CoA thioesterases and their derived lipid mediators: linking Acyl-CoA metabolic reprogramming to the tumor microenvironment.Frontiers in immunology · 2026Review
- Piperine: From Green Extraction to Clinical Translation-A Review.Drug design, development and therapy · 2026Review
- Therapeutic potential and mechanisms of plant metabolites in endometritis: a comprehensive review.Frontiers in pharmacology · 2026Review
- Moxibustion Promotes Tumor Immune Responses and Vascular Normalization to Enhance Antitumor Effect of Cisplatin in Lewis Lung Carcinoma Mice.Integrative cancer therapiesArticle
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Authors and funding
11 authors.
Funding
Abstract
backgroundPiper longum demonstrates significant therapeutic potential against gastric cancer (GC), but its underlying mechanisms remain incompletely understood. This study aimed to establish a comprehensive multi-omics framework to elucidate Piper longum's anti-cancer mechanisms.
methodsWe integrated in vivo experiments, metabolomics, gut microbiota analysis, mass spectrometry, and network pharmacology to investigate Piper longum's effects. In vivo studies assessed its dose-dependent inhibition of GC growth compared to standard chemotherapy (L-OHP + 5-FU). Metabolomics identified altered lipid metabolism pathways, while gut microbiota analysis evaluated its impact on microbial composition. Piperine was identified as the key active compound, and ACOT1 was pinpointed as a critical molecular target through integrated analysis.
resultsPiper longum significantly suppressed gastric cancer (GC) growth in a dose-dependent manner, with high-dose treatment demonstrating superior efficacy compared to conventional chemotherapy (L-OHP + 5-FU). Metabolomic analysis revealed that its anti-cancer mechanism primarily involves the reprogramming of lipid metabolism pathways in GC cells, while gut microbiota assessment confirmed that it modulates intestinal flora composition without compromising microbial diversity, supporting its favorable safety profile. Mass spectrometry identified piperine as the key bioactive compound, and integrated metabolomics and network pharmacology further pinpointed ACOT1 as a critical molecular target, which interacts with piperine that confirmed by CETSA. Notably, high ACOT1 expression was associated with poor prognosis in GC patients, underscoring its therapeutic relevance.
conclusionsThis study elucidates Piper longum's "component-target-pathway" mechanism in GC treatment, highlighting piperine-ACOT1-de novo lipogenesis regulatory pathway as a critical axis. Additionally, it establishes a robust multi-omics framework for evaluating traditional medicine efficacy, providing a theoretical foundation for Piper longum's clinical application in GC therapy.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.