Article in Angewandte Chemie (International ed. in English), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
2 · The registry
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Mariah A Cook *Department of Chemistry and Biochemistry, The University of Texas at Dallas, 800 West Campbell Road, Richardson, TX, 75080, USA.ORCID 0009-0008-6663-6833
Jonathan D Smailys *Department of Molecular Biosciences, University of Texas, 100 East 24th Street, Austin, TX, 78712, USA.
Ke Ji *Department of Chemistry and Biochemistry, The University of Texas at Dallas, 800 West Campbell Road, Richardson, TX, 75080, USA.ORCID 0000-0002-0085-9646
Shelby M PhelpsDepartment of Chemistry and Biochemistry, The University of Texas at Dallas, 800 West Campbell Road, Richardson, TX, 75080, USA.ORCID 0000-0002-2885-189X
Jasmine N TutolDepartment of Chemistry and Biochemistry, The University of Texas at Dallas, 800 West Campbell Road, Richardson, TX, 75080, USA.ORCID 0000-0001-8493-4203
Wantae KimMcKetta Department of Chemical Engineering, University of Texas, 200 East Dean Keeton Street, Austin, TX, 78712, USA.
Whitney S Y OngDepartment of Chemistry and Biochemistry, The University of Texas at Dallas, 800 West Campbell Road, Richardson, TX, 75080, USA.ORCID 0000-0001-9821-9972
Weicheng PengDepartment of Chemistry and Biochemistry, The University of Texas at Dallas, 800 West Campbell Road, Richardson, TX, 75080, USA.ORCID 0000-0002-1481-625X
Caden MaydewDepartment of Chemistry and Biochemistry, The University of Texas at Dallas, 800 West Campbell Road, Richardson, TX, 75080, USA.ORCID 0000-0002-7579-6109
Y Jessie ZhangDepartment of Molecular Biosciences, University of Texas, 100 East 24th Street, Austin, TX, 78712, USA.ORCID 0000-0002-9360-5388
Sheel C DodaniDepartment of Chemistry and Biochemistry, The University of Texas at Dallas, 800 West Campbell Road, Richardson, TX, 75080, USA.ORCID 0000-0003-0271-6080
Funding
Optical Imaging Tools for Elucidating the Roles of Anions and Anionic Modifications in Cellular SignalingR35GM128923 · NIGMS · UNIVERSITY OF TEXAS DALLAS · PI Sheel Dodani · 2018 to 2026
$3.4M
Deciphering the phosphorylation pattern of RNA polymerase II for eukaryotic transcriptionR35GM148356 · NIGMS · UNIVERSITY OF TEXAS AT AUSTIN · PI Yan Jessie Zhang · 2023 to 2026
$2.3M
DO HIGH DOSE B VITAMINS DELAY AGE-RELATED DECAYR21AT001918 · NCCIH · CHILDREN'S HOSPITAL &RES CTR AT OAKLAND · PI AMES, BRUCE N · 2004 to 2005
$400k
National Institute of General Medical Sciences of the National Institutes of Health R35GM128923National Institute of General Medical Sciences of the National Institutes of Health R35GM148356National Science Foundation 2240095NCCIH NIH HHS R21 AT001918NIGMS NIH HHS R35 GM128923NIGMS NIH HHS R35 GM148356United Kingdom's Diamond Light Source AU36008U.S. Department of Energy Office of Science by Argonne National Laboratory DE-AC02-06CH11357Welch Foundation AT-1918-20170325Welch Foundation AT-2060-20210327Welch Foundation AT-2060-20240404
6 · The paper itself
Abstract
The duality of nitrate is nowhere better exemplified than in human physiology-a detrimental pollutant but also a protective nutrient-particularly as connected to nitric oxide. Aside from limited insights into nitrate uptake and storage, foundational nitrate biology has lagged. Genetically encoded fluorescent biosensors can address this gap with real-time imaging, but such technologies for mammalian cell applications remain rare. Here, we designed and engineered a biosensor fusing the green fluorescent protein EGFP and the nitrate recognition domain NreA from Staphylococcus carnosus. Seven rounds of directed evolution and 15 mutations resulted in NitrOFF. NitrOFF has a high degree of allosteric communication between the domains reflected in a turn-off intensiometric response (K
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.
NitrOFF: An Engineered Fluorescent Biosensor to Illuminate Nitrate Transport in Living Cells. · full record | Socratic