Evidence map›Paper›PMID 40857621›Full record

ReviewChembiochem : a European journal of chemical biology2025

Aspartimide Formation and Its Prevention in Fmoc Chemistry Solid Phase Peptide Synthesis.

Marco J W Kong, Tim J H P van den Braak, Kevin Neumann

Abstract readReview
PubMed Full text at PMC Publisher
In one paragraph

Review in Chembiochem : a European journal of chemical biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Review
  3. Aspartimide Formation and Its Prevention in Fmoc Chemistry Solid Phase Peptide Synthesis.Chembiochem : a European journal of chemical biology · 2025
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Marco J W KongInstitute for Molecules and Materials, Radboud University, Heyendaalseweg 135, Nijmegen, 6525, AJ, Netherlands.ORCID https://orcid.org/0009-0004-4269-7472
Tim J H P van den BraakInstitute for Molecules and Materials, Radboud University, Heyendaalseweg 135, Nijmegen, 6525, AJ, Netherlands.
Kevin NeumannInstitute for Molecules and Materials, Radboud University, Heyendaalseweg 135, Nijmegen, 6525, AJ, Netherlands.ORCID https://orcid.org/0000-0002-6683-0774

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Aspartimide formation remains a persistent challenge during Fmoc chemistry solid phase peptide synthesis. This review outlines various strategies to suppress base-mediated aspartimide formation, including the use of ester β-carboxyl protecting groups, non-ester-based β-carboxyl masking groups, and backbone-protecting groups. In addition, alternatives to the Fmoc group are explored that are cleavable under nonbasic conditions. The work discussed in this review highlights that the continued development of compatible and scalable tactics toward aspartimide prevention is essential for advancing peptide synthesis in both research and industry.

Indexed as

Amino AcidsFluorenesPeptidesSolid-Phase Synthesis TechniquesAspartic AcidAmino AcidsAspartic AcidaspartimideFluorenesN(alpha)-fluorenylmethyloxycarbonylamino acidsPeptidesaspartic acidaspartimidefmoc chemistrypeptide synthesissolid phase peptide synthesis

Identifiers

PMID40857621
PMCPMC12447389

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.