ArticleJournal of inflammation research2025
Blockade of the Notch Pathway by Inhibition of Fucosylation Ameliorates Chondrocyte Inflammation and Extracellular Matrix Degradation.
Article in Journal of inflammation research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Objective: Osteoarthritis is a chronic degenerative disease characterised by damage to articular cartilage and degradation of the chondrocyte matrix. It has been shown that inflammation occurs with a variety of protein glycosylation abnormalities, among which elevated levels of fucosylation is one of the characteristics of OA. Now, the relationship between fucosylation and OA is not clear. By summarizing, we infer that elevated levels of fucosylation may affect the course of OA, possibly through the Notch signalling pathway. Methods: In in vitro experiments, we established a cellular inflammation model on a mature human chondrocyte cell line and intervened with the inhibitor SGN-2FF. The effect of decreased levels of fucosylation on chondrocyte apoptosis was observed by methods such as Calcein-AM/PI staining and Lectin blotting. By western bolt and immunofluorescence, it was verified that the decreased level of fucosylation could affect the activity of Notch signalling pathway and reduce the release of inflammatory factors, thus alleviating inflammation. In in vivo experiments, OA rats were treated by joint cavity injection of SGN-2FF, and the treatment status was assessed by ELISA and Micro-CT analysis. Results: We found abnormally elevated levels of fucosylation in a model of chondrocyte inflammation. Upon inhibition of fucosylation using SGN-2FF, there was a decrease in the expression of inflammation-related factors and a decrease in the number of apoptotic cells. With the decrease of fucosylation, the expression of activated Notch1 was significantly reduced, hindering the activation of Notch pathway and reducing the release of pro-inflammatory factors. Animal experiments showed that inhibition of fucoidan glycosylation could effectively reduce the expression of IL-6 and TNF-α, reduce cartilage damage and alleviate OA. Conclusion: Inhibition of fucosylation can ameliorate chondrocyte inflammation and extracellular matrix degradation by decreasing Notch1 activity and down-regulating the expression of the Notch pathway, which can effectively alleviate the development of OA.
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