Evidence map›Paper›PMID 40861481›Full record

ArticleFrontiers in cellular and infection microbiology2025

Puf4 -mediated oxidative stress virulence attenuation in

Chenhao Suo, Jianjun Lei, Wanli Zhang, Lihui Jia, He Zhang

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Frontiers in cellular and infection microbiology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. L-cysteine inhibited the growth ofApplied and environmental microbiology · 2026
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Chenhao SuoDepartment of Laboratory Animal Center, General Hospital of Northern Theater Command, Shenyang, Liaoning, China.
Jianjun LeiDepartment of Laboratory Animal Center, General Hospital of Northern Theater Command, Shenyang, Liaoning, China.
Wanli ZhangCollege of Life and Health Science, Northeastern University, Shenyang, Liaoning, China.
Lihui JiaDepartment of Medical Security Center, General Hospital of Northern Theater Command, Shenyang, Liaoning, China.
He ZhangDepartment of Laboratory Animal Center, General Hospital of Northern Theater Command, Shenyang, Liaoning, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Introduction: Methods: In this study, we systematically investigated the role of Puf4 in the metabolism and virulence of Results: Colony-spotting assay assays show that Puf4 affects the cell membrane and oxidative stress. RNA-seq analysis revealed that Puf4 was enriched in key metabolic pathways, including carbohydrate metabolism, oxidative phosphorylation, and glycolysis. Biochemical analysis revealed that Puf4 overexpression led to significant increases in both total carbohydrate and glycogen content. Additionally, the Puf4-overexpressing strain showed elevated ROS levels, and reduced resistance to antimycin A, indicating decreased oxidative stress tolerance. Notably, Puf4 overexpression resulted in reduced capsule size and decreased L-DOPA production, accompanied by downregulated expression of the capsule-associated gene Discussion: These findings suggest that Puf4 may serve as a regulator linking metabolic status and pathogenic potential in

Indexed as

Cryptococcus neoformansFungal ProteinsOxidative StressRNA-Binding ProteinsAnimalsCarbohydrate MetabolismCell WallCryptococcosisGene Expression Regulation, FungalMelaninsMiceMice, Inbred BALB CReactive Oxygen SpeciesVirulenceFungal ProteinsMelaninsReactive Oxygen SpeciesRNA-Binding Proteinscarbohydrate metabolismCryptococcus neoformansfungal virulenceoxidative stressRNA-binding protein

Identifiers

PMID40861481
PMCPMC12375554

What Socratic holds

Textmetadata
LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.