ArticleInvestigative ophthalmology & visual science2025
Open-Field Blast Injury Disrupts Corneal Gene Expression Linked to Ion Transport, Sensory Perception, and Neural Signaling.
Article in Investigative ophthalmology & visual science, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
1 citing paper in PubMed.
- Comparison of explosively driven shock tube and open-air blast wave propagation.Scientific reports · 2026Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors.
Funding
Abstract
Purpose: Open-field blast injury (OFBI) is a common cause of vision loss. This study investigated OFBI-induced changes in corneal transcriptome, signaling pathways, and protein-protein interactions using RNA sequencing (RNA-seq). Methods: Sixty-four C57BL/6J mice were used. OFBI was produced in live mice with 350 g of Composition C4 explosive at a 3 meters (m) standoff distance. RNA was extracted from OFBI-exposed and unexposed corneas, and RNA-seq libraries were generated and sequenced on the Illumina NovaSeq 6000 platform. Reads were aligned to the mouse reference GRCm38 genome using HISAT2. Differential gene expression (DGE) analysis was performed using DESeq2. Pathway enrichments were studied using Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), and Reactome databases. Protein-protein interaction (PPI) networks were constructed using STRING database and Cytoscape coupled with the cytoHubba plugin. Results: DGE analysis identified 2860 significantly DGEs in OFBI-exposed mice from unexposed (adjusted P < 0.05) mice. The 30 upregulated and 735 downregulated genes showing a log2 fold change ≥ ±1.5 were identified. Enriched pathways included ion channel and membrane potential regulation, sensory perception, and neurotransmitter signaling. The Grik2, Gabrg3, Drd2, Gabrr1, Gabra2, and Drd4 genes which are commonly associated with ion channel regulation, neuronal signaling, and sensory functions were significantly downregulated on day 14 post-OFBI. The three distinct PPI pathways were associated with ion channels, sensory perception, and neurotransmitter transport. RNA sequence and gene count files are published at the NCBI Gene Expression Omnibus (GSE292886). Conclusions: OFBI led to significant disruptions in corneal transcriptome critical in the modulation of ion channel activity, sensory perception, and neurotransmitter signaling.
Indexed as
Identifiers
What Socratic holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.