Evidence map›Paper›PMID 40863737›Full record

ArticleMethods and protocols2025

Protocol for the Systematic Quantitative Ultrastructural Analysis of Mitochondria in Cardiac Tissue.

Rebecca Schönmehl, Lina Winter, Daniel H Mendelsohn, Wing-Hoi Cheung, Ronald Man Yeung Wong, Steffen Pabel, Samuel Sossalla, Christoph Brochhausen

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods and protocols, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Rebecca SchönmehlInstitute of Pathology, Medical Faculty Mannheim, Heidelberg University, 68167 Mannheim, Germany.ORCID 0009-0007-3358-6174
Lina WinterInstitute of Pathology, Medical Faculty Mannheim, Heidelberg University, 68167 Mannheim, Germany.
Daniel H MendelsohnDepartment of Neurology, LMU University Hospital, LMU Munich, 81377 Munich, Germany.
Wing-Hoi CheungDepartment of Orthopaedics and Traumatology, The Chinese University of Hong Kong, Hong Kong, China.
Ronald Man Yeung WongDepartment of Orthopaedics and Traumatology, The Chinese University of Hong Kong, Hong Kong, China.ORCID 0000-0001-5615-6627
Steffen PabelDepartment of Internal Medicine II, University Medical Center Regensburg, 93053 Regensburg, Germany.
Samuel SossallaGerman Center for Cardiovascular Research (DZHK), Partner Site Rhein Main, 61231 Bad Nauheim, Germany.ORCID 0000-0001-8034-2673
Christoph BrochhausenInstitute of Pathology, Medical Faculty Mannheim, Heidelberg University, 68167 Mannheim, Germany.ORCID 0000-0002-6761-0247

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Mitochondria play a crucial role in adapting to fluctuating energy demands, particularly in various heart diseases. In addition to functional analyses such as the measurement of ROS or ATP, analysis of mitochondrial ultrastructure can be used to draw further conclusions about their functions and effects in tissue. In this protocol, we introduce a set of measurements to compare the ultrastructural and functional characteristics of human left ventricular mitochondria, using transmission electron microscopy (TEM). Measured parameters included mean size in µm

Indexed as

cardiomyocyteselectron microscopymitochondriaquantitative image analysisultrastructure

Identifiers

PMID40863737
PMCPMC12388055

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.