Evidence map›Paper›PMID 40865380›Full record

ArticlePoultry science2025

Pathogen purification and genetic characterization of SPF BWEL chicken population.

Caixia Gao, Haibo Yu, Wei Zhang, Changwen Li, Hongyan Chen, Changyou Xia

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Poultry science, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Caixia GaoState Key Laboratory for Animal Disease Control and Prevention, Heilongjiang Provincial Key Laboratory of Laboratory Animal and Comparative Medicine, National Poultry Laboratory Animal Resource Center, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences (CAAS), Harbin, 150069, China.
Haibo YuState Key Laboratory for Animal Disease Control and Prevention, Heilongjiang Provincial Key Laboratory of Laboratory Animal and Comparative Medicine, National Poultry Laboratory Animal Resource Center, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences (CAAS), Harbin, 150069, China.
Wei ZhangState Key Laboratory for Animal Disease Control and Prevention, Heilongjiang Provincial Key Laboratory of Laboratory Animal and Comparative Medicine, National Poultry Laboratory Animal Resource Center, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences (CAAS), Harbin, 150069, China.
Changwen LiState Key Laboratory for Animal Disease Control and Prevention, Heilongjiang Provincial Key Laboratory of Laboratory Animal and Comparative Medicine, National Poultry Laboratory Animal Resource Center, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences (CAAS), Harbin, 150069, China.
Hongyan ChenState Key Laboratory for Animal Disease Control and Prevention, Heilongjiang Provincial Key Laboratory of Laboratory Animal and Comparative Medicine, National Poultry Laboratory Animal Resource Center, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences (CAAS), Harbin, 150069, China.
Changyou XiaState Key Laboratory for Animal Disease Control and Prevention, Heilongjiang Provincial Key Laboratory of Laboratory Animal and Comparative Medicine, National Poultry Laboratory Animal Resource Center, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences (CAAS), Harbin, 150069, China. Electronic address: xiachangyou@caas.cn.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Specific pathogen free (SPF) chickens are crucial biological resources for fundamental research and industrial development in biomedicine, pharmaceutical sciences, and disease prevention and control. China achieved a significant milestone in 2000 by establishing its first independently developed SPF BWEL chicken population with proprietary intellectual property rights. During SPF chicken population development, rigorous quality control of microorganism, genetics, and environment are crucial for maintaining research-grade standards. In this study, we implemented regular monitoring of 20 specified avian pathogens and established comprehensive surveillance strategies to maintain pathogen-free status. Simultaneously, nine critical isolation environment parameters were systematically monitored to maintain optimal containment conditions. Furthermore, we performed SNP genotyping using chicken breeding chip on 262 F28 generation SPF BWEL chickens, followed by population structure analysis through kinship evaluation and clustering to reconstruct family lineages. Runs of homozygosity (ROH) were statistically analyzed to calculate individual and population-level inbreeding coefficients. The results showed that successful exclusion of 20 specific pathogens exceeding the National Standard of the People's Republic of China requirements, confirming excellent microbial quality. Meanwhile, all isolation parameters also complied with the provisions of the National Standard, ensuring environmental stability. Genetic analyses revealed the population could be clustered into five distinct families and the average inbreeding coefficient based on ROH (F

Indexed as

ChickensPoultry DiseasesAnimal HusbandryAnimalsBreedingChinaFemaleGenotypePolymorphism, Single NucleotideSpecific Pathogen-Free OrganismsGroup familyInbreeding coefficientMicrobial qualitySNPSPF chicken

Identifiers

PMID40865380
PMCPMC12683126

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.