ArticleNature communications2025
Widespread epistasis shapes RNA polymerase II active site function and evolution.
Article in Nature communications, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
6 citing papers in PubMed.
- Glc7/PP1 triggers Paf1 complex dissociation from RNA polymerase II to enable transcription termination.Genes & development · 2026Article
- Glc7/PP1 triggers Paf1 complex dissociation from RNA polymerase II to enable transcription termination.bioRxiv : the preprint server for biology · 2025Article
- Widespread epistasis shapes RNA polymerase II active site function and evolution.Nature communications · 2025Article
- RNA Polymerase II Activity Control of Gene Expression and Involvement in Disease.Journal of molecular biology · 2025Review
- Article
- Higher-order epistasis within Pol II trigger loop haplotypes.bioRxiv : the preprint server for biology · 2024Article
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4 authors.
Funding
Abstract
Multi-subunit RNA Polymerases are responsible for transcription in all kingdoms of life. These enzymes rely on dynamic, highly conserved active site domains such as the so-called "trigger loop" to accomplish steps in the transcription cycle. Mutations in the RNA polymerase II trigger loop confer a spectrum of biochemical and genetic phenotypes that suggest two main classes, which decrease or increase catalysis or other nucleotide addition cycle events. The RNA polymerase II active site relies on networks of residue interactions to function, and mutations likely perturb these networks in ways that may alter mechanisms. Here, we take a structural genetics approach to reveal residue interactions within and surrounding the RNA polymerase II trigger loop - determining its "interaction landscape" - by deep mutational scanning in Saccharomyces cerevisiae RNA polymerase II. This analysis reveals connections between trigger loop residues and surrounding domains, demonstrating that trigger loop function is tightly coupled to its specific enzyme context.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.