Evidence map›Paper›PMID 40875124›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

A Vivo-Morpholino-Based Approach to Block Paralog-Specific SUMOylation.

Rebeca Orozco-Sepúlveda, Andrea García-Morin, Isabel Gutiérrez-Zubiate, Germán Rosas-Acosta

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Rebeca Orozco-SepúlvedaThe University of Texas at El Paso (UTEP), El Paso, TX, USA.
Andrea García-MorinThe University of Texas at El Paso (UTEP), El Paso, TX, USA.
Isabel Gutiérrez-ZubiateThe University of Texas at El Paso (UTEP), El Paso, TX, USA.
Germán Rosas-AcostaThe University of Texas at El Paso (UTEP), El Paso, TX, USA. grosas3@utep.edu.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

We present an innovative method to specifically decrease SUMO2 SUMOylation by using a set of vivo-morpholinos inducing an alternative splicing event that produces a mRNA coding for SUMO2alpha, a non-conjugatable form of SUMO2. This method, also applicable to target SUMO1 and SUMO3, provides an alternative approach to assess the function of the various SUMO paralogs in different cells and the SUMO paralog preference of any given SUMO target.

Indexed as

MorpholinosSmall Ubiquitin-Related Modifier ProteinsSumoylationAlternative SplicingAnimalsHumansRNA, MessengerSUMO-1 ProteinMorpholinosRNA, MessengerSmall Ubiquitin-Related Modifier ProteinsSUMO-1 ProteinSUMO2 protein, humanAlternative splicingExon-skippingMorpholinoNon-conjugatableSUMO2SUMO2𝛼Vivo-morpholino

Identifiers

PMID40875124

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.