Evidence map›Paper›PMID 40877108›Full record

ArticleBritish journal of anaesthesia2025

Human neutrophil-derived extracellular vesicles induce renal endothelial inflammation in critical illness: an ex vivo investigation.

Jonny R Stephens, Yoichi Iki, Tomoaki Yasuda, Elizabeth Brown, Diianeira Tsiridou, Ashleigh Green, Parind Patel, Umeer Waheed, Richard Stümpfle, Aruni Ratnayake and 9 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in British journal of anaesthesia, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Observational
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

19 authors.

Jonny R StephensDivision of Anaesthetics, Pain Medicine and Intensive Care, Department of Surgery and Cancer, Imperial College London, London, UK.
Yoichi IkiDivision of Anaesthetics, Pain Medicine and Intensive Care, Department of Surgery and Cancer, Imperial College London, London, UK; Department of Intensive Care Medicine, Institute of Science Tokyo, Tokyo, Japan.
Tomoaki YasudaDivision of Anaesthetics, Pain Medicine and Intensive Care, Department of Surgery and Cancer, Imperial College London, London, UK.
Elizabeth BrownDivision of Anaesthetics, Pain Medicine and Intensive Care, Department of Surgery and Cancer, Imperial College London, London, UK.
Diianeira TsiridouDivision of Anaesthetics, Pain Medicine and Intensive Care, Department of Surgery and Cancer, Imperial College London, London, UK.
Ashleigh GreenDivision of Anaesthetics, Pain Medicine and Intensive Care, Department of Surgery and Cancer, Imperial College London, London, UK.
Parind PatelDivision of Anaesthetics, Pain Medicine and Intensive Care, Department of Surgery and Cancer, Imperial College London, London, UK; Department of Critical Care, Hammersmith Hospital, Imperial College Healthcare NHS Trust, London, UK.
Umeer WaheedDepartment of Critical Care, Hammersmith Hospital, Imperial College Healthcare NHS Trust, London, UK.
Richard StümpfleDepartment of Critical Care, Hammersmith Hospital, Imperial College Healthcare NHS Trust, London, UK.
Aruni RatnayakeCentre for Inflammatory Disease, Department of Inflammation and Immunology, Imperial College London, London, UK.
Padmini SarathchandraNational Heart and Lung Institute, Imperial College London, Harefield Hospital, London, UK.
Xingzhi ChengDivision of Anaesthetics, Pain Medicine and Intensive Care, Department of Surgery and Cancer, Imperial College London, London, UK.
Anthony GordonDivision of Anaesthetics, Pain Medicine and Intensive Care, Department of Surgery and Cancer, Imperial College London, London, UK.
Kenji WakabayashiDepartment of Intensive Care Medicine, Institute of Science Tokyo, Tokyo, Japan.
Kieran O'DeaDivision of Anaesthetics, Pain Medicine and Intensive Care, Department of Surgery and Cancer, Imperial College London, London, UK.
Frederick W K TamCentre for Inflammatory Disease, Department of Inflammation and Immunology, Imperial College London, London, UK.
Stephen J BrettDivision of Anaesthetics, Pain Medicine and Intensive Care, Department of Surgery and Cancer, Imperial College London, London, UK; Department of Critical Care, Hammersmith Hospital, Imperial College Healthcare NHS Trust, London, UK.
Masao TakataDivision of Anaesthetics, Pain Medicine and Intensive Care, Department of Surgery and Cancer, Imperial College London, London, UK.
Sanooj SoniDivision of Anaesthetics, Pain Medicine and Intensive Care, Department of Surgery and Cancer, Imperial College London, London, UK; Department of Critical Care, Hammersmith Hospital, Imperial College Healthcare NHS Trust, London, UK. Electronic address: s.soni@imperial.ac.uk.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundCirculating neutrophil-derived extracellular vesicles (NEVs) may contribute to the pathophysiology of acute kidney injury by causing glomerular endothelial inflammation.

methodsNEVs were first isolated from ex vivo, lipopolysaccharide stimulation of whole blood taken from healthy volunteers (median age [interquartile range {IQR}]: 32 [26-42] yr; 47% female), and also from plasma of COVID-19 patients with acute respiratory distress syndrome (median age [IQR]: 59 [52-66] yr; 45% female). NEVs were incubated for 4 h in a co-culture of peripheral blood mononuclear cells and either human umbilical vein endothelial cells or renal glomerular endothelial cells. Enzyme-linked immunoassays (tumour necrosis factor-alpha [TNF]) and flow cytometry (median fluorescence intensity [MFI]) were used to quantify cell-specific markers of inflammation/activation, in the presence/absence of pharmacological inhibitors.

resultsNEVs were internalised by monocytes, leading to their activation via the p38 mitogen-activated protein kinase pathway and increased release of TNF (median [IQR]: 676 [474-1731] pg ml

conclusionsCirculating NEVs may contribute to acute kidney injury through renal endothelial inflammation in a monocyte-dependent fashion in patients with acute respiratory distress syndrome.

Indexed as

Acute Kidney InjuryExtracellular VesiclesNeutrophilsAdultAgedCoculture TechniquesCOVID-19Critical IllnessEndothelial CellsFemaleHumansHuman Umbilical Vein Endothelial CellsInflammationMaleMiddle AgedRespiratory Distress SyndromeTumor Necrosis Factor-alphaCOVID-19critical illnessextracellular vesiclematrix metalloproteinase (MMP)renal glomerular inflammation

Identifiers

PMID40877108
PMCPMC12674056

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.