Evidence map›Paper›PMID 40877147›Full record

ArticleIn vivo (Athens, Greece)

Quantification of

Alejandro Mena Acra, Hiroshi Sakagami, Shin Uota, Masaaki Yoshihara, Shinji Kito, Maki Izawa, Yusei Ohtaka, Giichirou Nakaya, Yukari Koga-Ogawa, Tadamasa Nobesawa and 2 more

Abstract read
In one paragraph

Article in In vivo (Athens, Greece). The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Alejandro Mena Acra *Meikai University Research Institute of Odontology (M-RIO), Saitama, Japan.
Hiroshi Sakagami *Meikai University Research Institute of Odontology (M-RIO), Saitama, Japan; sakagami@dent.meikai.ac.jp.
Shin UotaMeikai University Research Institute of Odontology (M-RIO), Saitama, Japan.
Masaaki YoshiharaNippon Sunshow Medicine Manufacture Co. Ltd., Nagoya, Japan.
Shinji KitoDivision of Dental Radiology, Meikai University School of Dentistry, Saitama, Japan.
Maki IzawaDivision of Dental Radiology, Meikai University School of Dentistry, Saitama, Japan.
Yusei OhtakaDivision of Dental Radiology, Meikai University School of Dentistry, Saitama, Japan.
Giichirou NakayaFaculty of Health Sciences, Nihon Institute of Medical Science, Saitama, Japan.
Yukari Koga-OgawaFaculty of Health Sciences, Nihon Institute of Medical Science, Saitama, Japan.
Tadamasa NobesawaFaculty of Health Sciences, Nihon Institute of Medical Science, Saitama, Japan.
Daisuke UedaFaculty of Health Sciences, Nihon Institute of Medical Science, Saitama, Japan.
Ryuichiro SuzukiFaculty of Pharmacy and Pharmaceutical Sciences, Josai University, Saitama, Japan.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

BACKGROUND/

aimWith an increasing number of comorbid diseases, the number of drug intake by elderly people and their chances to X-ray exposure inevitably increase. However, it is unclear how these treatments affect longevity. Primary human diploid fibroblasts with limited life span have been used as a model system for the study of cellular senescence. Since MATERIALS AND

methodsCells were treated for 14 days with or without (as control) various concentrations of test samples in culture medium supplemented with fetal bovine serum (FBS), without medium changes, but overlayed with fresh medium to minimize the cell detachment and nutritional deprivation. From the dose-response curve, the elongation index of the survival time (EI) was calculated by the ratio of maximum viable cell number at the optimal concentration of the test sample to that of the control at Day 14.

resultsIn general, six human fibroblasts prepared from dermal, oral and lung tissues required higher concentrations of FBS than cancer cells. Dermal fibroblasts were selected as the target cells, based on their higher hormetic responses. Quercetin, hydrocortisone, sodium ascorbate, vanillic acid and vanillin showed higher EI values than estradiol, curcumin, resveratrol, phellamurin, sodium butylate and X-ray irradiation, but did not reach the levels of plant extracts.

conclusionThe present method may be useful to quantitate the RLE activity of external and internal factors alone or in combination, in the presence of an appropriate positive control.

Indexed as

Biological ProductsCellular SenescenceFibroblastsCell ProliferationCells, CulturedCell SurvivalHumansBiological ProductsAnti-agingantioxidantshormones“overlay” methodplant extractsquantificationquercetinreplicative life spanX-ray

Identifiers

PMID40877147
PMCPMC12396065

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.