ArticleScientific reports2025
Cell-Free bone regeneration using dental MSCs secretomes from pulp and gingiva in rabbit tibial defects.
Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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Who cites it
3 citing papers in PubMed.
- The role of bioactive molecules in enhancing bone healing in oral and maxillofacial surgery: a review of current approaches and future directions.Oral and maxillofacial surgery · 2026Review
- Effects of mesenchymal stem cell secretome on osteoclast- and osteogenesis-related markers during orthodontic relapse.Journal of Taibah University Medical Sciences · 2026Article
- Sustainable nanomaterials for precision dental medicine: green synthesis, therapeutic applications, and future directions.Journal of nanobiotechnology · 2026Review
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Authors and funding
8 authors.
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Abstract
Mesenchymal stem cells (MSCs)-secretome represent a promising cell-free strategy for bone regeneration, overcoming cell therapies' drawbacks. This study compares the bone repair capabilities of secretomes derived from human dental pulp stem cells (hDPSCs) and human gingival stem cells (hGMSCs) in a rabbit tibial defect model. Secretomes were prepared, and levels of RUNX, osterix, and alkaline phosphatase (ALP) levels were quantified using ELISA. Six-mm defects were created in rabbit tibiae treated with either hDPSCs or hGMSCs secretomes, collagen scaffolds, or left untreated. The mineral apposition rate (MAR) was assessed using fluorescent labeling. Histomorphometric analysis (including bone area percentage, mature/immature bone and bone marrow quantification) and qRT-PCR for osteocalcin were conducted at 3 and 6 weeks. Results showed significantly higher concentrations of RUNX, osterix, and ALP in hDPSCs secretome compared to hGMSCs. Additionally, defects treated with hDPSCs secretome exhibited a higher MAR and greater new bone formation, accelerated maturation (higher mature bone area; p < 0.05), and reduced bone marrow spaces at 3 weeks than those treated with hGMSCs, collagen, or control groups. By 6 weeks, both secretomes achieved comparable bone maturation (83-85% bone area), significantly surpassing controls. Both secretomes upregulated osteocalcin gene expression. These findings show the therapeutic potential of dental MSCs secretomes, and particularly hDPSCs secretomes, as a cell-free, clinically relevant method for improving bone regeneration. This approach addresses limitations associated with traditional bone grafting and possibly presents a new pathway for regenerative therapies in dentistry and orthopedics.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.