ArticleMicroscopy research and technique2025
Illuminating Satellite Cells: Light Sheet Fluorescence Microscopy for 3D Imaging of Murine Skeletal Muscles Damaged by Ex Vivo Forced Eccentric Contraction.
Article in Microscopy research and technique, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
2 citing papers in PubMed.
- Exercise stress and tissue remodeling: advances in exosome-mediated RNA-RBP networks in musculoskeletal injury repair and functional recovery.Frontiers in genetics · 2026Review
- Illuminating Satellite Cells: Light Sheet Fluorescence Microscopy for 3D Imaging of Murine Skeletal Muscles Damaged by Ex Vivo Forced Eccentric Contraction.Microscopy research and technique · 2025Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
12 authors.
Funding
Abstract
In this letter, we put forward the light sheet fluorescence microscopy (LSFM) as a cutting-edge tool for 3D imaging of whole skeletal muscle, focusing on satellite cells (SCs). SCs represent the resident adult muscle stem cells, normally lying quiescent between the sarcolemma of the myofiber and the surrounding basal lamina. They typically express Pax-7 and, when activated following damage, they sequentially express specific myogenic regulatory factors including the myogenic determination factor, MyoD, thus starting differentiation towards multinucleated myofibers to repair injured tissue. The present analysis was performed on an ex vivo model of murine skeletal muscle injured by a forced eccentric contraction in isometric condition. The entire muscles were subjected to a tissue clearing and whole-mount staining process, enabling optical access and specific labeling across the entire intact sample. We performed labeling either with a fluorescent analog of standard hematoxylin and eosin, or with specific immunostaining against Pax-7 and MyoD. This proof of concept study demonstrates the feasibility of whole-muscle imaging with LSFM for the evaluation of the spatial arrangement of resting and activated SCs, overcoming the methodological limits of conventional 2D histology. This innovative experimental pipeline can be useful to test novel therapeutic approaches aimed at enhancing tissue regeneration and other biomedical/clinical applications.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.