Evidence map›Paper›PMID 40879983›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

(PP)-InsP Affinity Probes for Target Characterization by Immunoblotting and Mass Spectrometry.

Jaime A Isern, Abhirup Majumdar, Annika Richter, Dorothea Fiedler

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Jaime A IsernLeibniz-Forschungsinstitut für Molekulare Pharmakologie (FMP), Berlin, Germany.
Abhirup MajumdarLeibniz-Forschungsinstitut für Molekulare Pharmakologie (FMP), Berlin, Germany.
Annika RichterLeibniz-Forschungsinstitut für Molekulare Pharmakologie (FMP), Berlin, Germany.
Dorothea FiedlerLeibniz-Forschungsinstitut für Molekulare Pharmakologie (FMP), Berlin, Germany. Fiedler@fmp-berlin.de.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Pulldown experiments isolate molecular interactions using a "bait" molecule on solid supports, often leveraging the biotin-streptavidin system. Here, a streamlined workflow is described, which employs biotinylated inositol phosphate (InsPs) and inositol pyrophosphate (PP-InsP) probes to enrich target proteins from complex proteomes. The reagents are first immobilized onto streptavidin-coated beads, then exposed to cell lysates, and subsequently washed to remove nonspecific interactions. The enriched proteins are then eluted and analyzed via western blot or quantitative mass spectrometry. This approach leverages biotin-tagged probes to enhance coupling efficiency, simplify workflows, and enable diverse applications.

Indexed as

Affinity LabelsImmunoblottingInositol PhosphatesMass SpectrometryProteomicsBiotinBiotinylationHumansStreptavidinAffinity LabelsBiotinInositol PhosphatesStreptavidinAffinity-based protein profiling (AfBPP)BiotinylationInositol phosphate affinity probeLabel-free quantificationMass spectrometryWestern blot

Identifiers

PMID40879983

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.