ArticleMethods in molecular biology (Clifton, N.J.)2025
Visualizing and Identifying Inositol Pyrophosphate Isomers in Crystallo.
Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Inositol pyrophosphates (PP-InsPs), which possess functionally significant diphosphate groups, regulate a wide range of biological processes across eukaryotic organisms. The presence and isomeric diversity of PP-InsPs suggest potential biological relevance and enable these molecules to serve as versatile regulators. Identification and characterization of the PP-InsP isomers require specialized approaches. We have successfully utilized X-ray crystallography to directly visualize and identify PP-InsP isomers, thereby informing biological studies and advancing our understanding of their functional roles. Here, we describe detailed methods for identifying PP-InsP isomers in Crystallo.
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