Evidence map›Paper›PMID 40898342›Full record

ArticleCell communication and signaling : CCS2025

Connexin hemichannel blockade by abEC1.1 disrupts glioblastoma progression, suppresses invasiveness, and reduces hyperexcitability in preclinical models.

Viola Donati, Chiara Di Pietro, Luca Persano, Elena Rampazzo, Mariateresa Panarelli, Clara Cambria, Anna Selimi, Lorenzo Manfreda, Ana Gabriela de Oliveira do Rêgo, Gina La Sala and 8 more

Abstract read
In one paragraph

Article in Cell communication and signaling : CCS, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Review
  2. Article
  3. Review
  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

18 authors.

Viola DonatiDepartment of Biomedical Sciences, University of Padua, Padua, 35131, Italy.
Chiara Di PietroCNR Institute of Biochemistry and Cell Biology, Monterotondo, Rome, 00015, Italy.
Luca PersanoDepartment of Women's and Children's Health, University of Padova, Padua, 35128, Italy.
Elena RampazzoDepartment of Women's and Children's Health, University of Padova, Padua, 35128, Italy.
Mariateresa PanarelliDepartment of Biomedical Sciences, University of Padua, Padua, 35131, Italy.
Clara CambriaDepartment of Medical Biotechnology and Translational Medicine (BIOMETRA), University of Milan, Milan, Italy.
Anna SelimiDepartment of Medical Biotechnology and Translational Medicine (BIOMETRA), University of Milan, Milan, Italy.
Lorenzo ManfredaDepartment of Women's and Children's Health, University of Padova, Padua, 35128, Italy.
Ana Gabriela de Oliveira do RêgoCNR Institute of Biochemistry and Cell Biology, Monterotondo, Rome, 00015, Italy.
Gina La SalaCNR Institute of Biochemistry and Cell Biology, Monterotondo, Rome, 00015, Italy.
Camilla SpregaCNR Institute of Biochemistry and Cell Biology, Monterotondo, Rome, 00015, Italy.
Arianna CalistriDepartment of Molecular Medicine, University of Padua, Padua, 35121, Italy.
Catalin Dacian CiubotaruCNR Istituto Officina Dei Materiali, Area Science Park Basovizza, S.S. 14, Km 163,5, Trieste, 34149, Italy.
Guang YangShanghai Institute for Advanced Immunochemical Studies, ShanghaiTech University, Shanghai, 201210, People's Republic of China.
Francesco ZontaDepartment of Biosciences and Bioinformatics, School of Science, Xi'an Jiaotong-Liverpool University, Suzhou, 215123, People's Republic of China.
Flavia AntonucciDepartment of Medical Biotechnology and Translational Medicine (BIOMETRA), University of Milan, Milan, Italy.
Daniela MarazzitiCNR Institute of Biochemistry and Cell Biology, Monterotondo, Rome, 00015, Italy. daniela.marazziti@cnr.it.
Fabio MammanoCNR Institute of Biochemistry and Cell Biology, Monterotondo, Rome, 00015, Italy. fabio.mammano@unipd.it.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundConnexin (Cx) hemichannels (HCs) contribute to glioblastoma (GBM) progression by facilitating intercellular communication and releasing pro-tumorigenic molecules, including ATP and glutamate.

methodsThe efficacy of abEC1.1, a monoclonal antibody that inhibits Cx26, Cx30, and Cx32 HCs, was assessed in vitro by measuring invasion capability, dye and Ca

resultsabEC1.1 suppressed GBM cell invasion, reducing gliotransmitter release, and impairing tumor progression. In patient-derived GBM cultures, abEC1.1 significantly decreased cell migration and ATP/glutamate release. In vivo, AAV-mediated antibody gene delivery or CED of the purified antibody reduced tumor burden and prolonged survival in the GL261 syngeneic mouse model of GBM. Furthermore, abEC1.1 mitigated glioma-induced excitatory synaptic activity in the 2D co-culture model, suggesting a dual role in tumor control and hyperexcitability suppression.

conclusionsOur findings establish Cx HC inhibition as a promising therapeutic avenue in GBM and highlight abEC1.1 as a potential candidate for clinical translation.

Indexed as

Antibodies, MonoclonalBrain NeoplasmsConnexinsDisease ProgressionGlioblastomaAdenosine TriphosphateAnimalsAstrocytesCell Line, TumorCell MovementDisease Models, AnimalHumansMiceMice, Inbred C57BLNeoplasm InvasivenessAdenosine TriphosphateAntibodies, MonoclonalConnexinsGL261 orthotopic syngeneic mouse modelGlioblastomaMonoclonal antibodyPatient-derived glioma cellsTargeted therapy

Identifiers

PMID40898342
PMCPMC12403430

What Socratic holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.