Evidence map›Paper›PMID 40901906›Full record

ArticlePlant biotechnology journal2025

Cold Stress-Induced (Z)-3-Hexenol and Thymol Enhance Cold Tolerance of Tea Plants by Activating Ca

Yuantao Liu, Yaling Song, Zhengwei Luo, Lisha Wang, Jieyang Jin, Tingting Jing, Mingyue Zhao, Qiang Wang, Wilfried Schwab, Meng Ye and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Plant biotechnology journal, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Yuantao LiuNational Key Laboratory for Tea Plant Germplasm Innovation and Resource Utilization, Anhui Agricultural University, Hefei, China.
Yaling SongNational Key Laboratory for Tea Plant Germplasm Innovation and Resource Utilization, Anhui Agricultural University, Hefei, China.
Zhengwei LuoNational Key Laboratory for Tea Plant Germplasm Innovation and Resource Utilization, Anhui Agricultural University, Hefei, China.
Lisha WangNational Key Laboratory for Tea Plant Germplasm Innovation and Resource Utilization, Anhui Agricultural University, Hefei, China.
Jieyang JinNational Key Laboratory for Tea Plant Germplasm Innovation and Resource Utilization, Anhui Agricultural University, Hefei, China.
Tingting JingNational Key Laboratory for Tea Plant Germplasm Innovation and Resource Utilization, Anhui Agricultural University, Hefei, China.
Mingyue ZhaoNational Key Laboratory for Tea Plant Germplasm Innovation and Resource Utilization, Anhui Agricultural University, Hefei, China.
Qiang WangNational Key Laboratory for Tea Plant Germplasm Innovation and Resource Utilization, Anhui Agricultural University, Hefei, China.
Wilfried SchwabNational Key Laboratory for Tea Plant Germplasm Innovation and Resource Utilization, Anhui Agricultural University, Hefei, China.
Meng YeTea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou, China.ORCID https://orcid.org/0000-0002-6785-0099
Chuankui SongNational Key Laboratory for Tea Plant Germplasm Innovation and Resource Utilization, Anhui Agricultural University, Hefei, China.ORCID https://orcid.org/0000-0002-7852-1194

Funding

Elite Youth Program of Chinese Academy of Agricultural SciencesGerman Research Foundation DFG, SCHW 634/34 1National Key Research and Development Program of China 2022YFF1003103National Natural Science Foundation of China 32150017National Natural Science Foundation of China 32372775National Natural Science Foundation of China U22A20499National Natural Science Foundation of China U23A20213
6 · The paper itself

Abstract

Volatile organic compounds (VOCs) released under cold stress have emerged as important mediators of stress tolerance. However, the specific functional VOCs and the mechanisms through which they confer cold tolerance remain largely unknown. In this study, we established a Fluo-8-based calcium detection system in tea (Camellia sinensis) protoplasts to investigate the interplay between cold-induced VOCs, calcium signalling, and cold tolerance. We identified (Z)-3-hexenol and thymol as key VOCs that significantly enhance tea plant cold tolerance by activating cytosolic calcium signalling. These VOCs upregulated cold-responsive genes (CsICE1, CsCBF1, and CsCBF2), enhanced antioxidant enzyme activities (SOD and POD), and improved photosynthetic efficiency under cold stress. Furthermore, we revealed that CsCDPK4, a calcium-dependent protein kinase, acts as a key mediator of (Z)-3-hexenol and thymol-induced calcium signalling. Silencing CsCDPK4 abolished the beneficial effects of (Z)-3-hexenol and thymol, underscoring its critical role in translating calcium signals into physiological adaptations. Our findings provide a mechanistic framework linking VOC perception, calcium signalling, and cold stress tolerance, offering novel strategies for enhancing crop resilience against abiotic stress in the face of climate challenges.

Indexed as

Calcium SignalingCamellia sinensisCold-Shock ResponseHexanolsThymolCalciumCold TemperatureGene Expression Regulation, PlantPlant ProteinsVolatile Organic CompoundsCalciumHexanolsPlant ProteinsThymolVolatile Organic CompoundsCa2+ signallingcold stressFluo‐8tea plantsthymolVOCs(Z)‐3‐hexenol

Identifiers

PMID40901906
PMCPMC12665057

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.