Evidence map›Paper›PMID 40909647›Full record

ArticlebioRxiv : the preprint server for biology2025

A Cryptic Binding Pocket Regulates the Metal-Dependent Activity of Cas9.

Mohd Ahsan, Aakash Saha, Delisa Ramos, Isabel Strohkendl, Erin Skeens, George P Lisi, David W Taylor, Giulia Palermo

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Mohd AhsanDepartment of Bioengineering, University of California Riverside, 900 University Avenue, Riverside, CA 52512, United States.ORCID 0000-0001-7669-3921
Aakash SahaDepartment of Bioengineering, University of California Riverside, 900 University Avenue, Riverside, CA 52512, United States.ORCID 0000-0003-0776-9771
Delisa RamosDepartment of Molecular Biosciences, University of Texas at Austin, Austin, TX, USA.
Isabel StrohkendlDepartment of Molecular Biosciences, University of Texas at Austin, Austin, TX, USA.ORCID 0000-0002-6290-8685
Erin SkeensDepartment of Molecular Biology, Cell Biology & Biochemistry, Brown University, Providence, RI, United States.ORCID 0000-0002-0580-1291
George P LisiDepartment of Molecular Biology, Cell Biology & Biochemistry, Brown University, Providence, RI, United States.ORCID 0000-0001-8878-5655
David W TaylorDepartment of Molecular Biosciences, University of Texas at Austin, Austin, TX, USA.
Giulia PalermoDepartment of Bioengineering, University of California Riverside, 900 University Avenue, Riverside, CA 52512, United States.ORCID 0000-0003-1404-8737

Funding

Imaging macromolecular machines in gene regulationR35GM138348 · NIGMS · UNIVERSITY OF TEXAS AT AUSTIN · PI TAYLOR, DAVID W · 2020 to 2024
$1.9M
Investigating the metal-dependent function, allostery and inhibition of CRISPR-Cas9R01GM141329 · NIGMS · UNIVERSITY OF CALIFORNIA RIVERSIDE · PI PALERMO, GIULIA · 2021 to 2025
$1.7M
Studies of Allostery between Multi-domain Proteins and Nucleic Acid ComplexesR01GM136815 · NIGMS · YALE UNIVERSITY · PI BATISTA, VICTOR S · 2021 to 2024
$1.4M
NIGMS NIH HHS R01 GM136815NIGMS NIH HHS R01 GM141329NIGMS NIH HHS R35 GM138348
6 · The paper itself

Abstract

Cas9 is a metal-dependent nuclease that has revolutionized gene editing across diverse cells and organisms exhibiting varying ion uptake, metabolism, and concentrations. However, how divalent metals impact its catalytic function, and consequently its editing efficiency in different cells, remains unclear. Here, extensive molecular simulations, Markov State Models, biochemical and NMR experiments, demonstrate that divalent metals - Mg

Identifiers

PMID40909647
PMCPMC12407680

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.