Evidence map›Paper›PMID 40914410›Full record

ArticleThe Journal of investigative dermatology2026

Regulated Release of Small Extracellular Vesicles Directs Neutrophil Recruitment in Cutaneous Wound Healing.

Wooil Choi, Dong Jun Park, Robert A Dorschner, Katie Pool, Sakeef Sayeed, Jenny Kezios, Jaebin Lee, Sebastian Adlawan, Brian P Eliceiri

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of investigative dermatology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Wooil ChoiDepartment of Surgery, University of California San Diego, La Jolla, California, USA.
Dong Jun ParkDepartment of Surgery, University of California San Diego, La Jolla, California, USA.
Robert A DorschnerDepartment of Dermatology, University of California San Diego, La Jolla, California, USA.
Katie PoolDepartment of Surgery, University of California San Diego, La Jolla, California, USA; Department of Dermatology, University of California San Diego, La Jolla, California, USA.
Sakeef SayeedDepartment of Surgery, University of California San Diego, La Jolla, California, USA.
Jenny KeziosDepartment of Surgery, University of California San Diego, La Jolla, California, USA.
Jaebin LeeDepartment of Surgery, University of California San Diego, La Jolla, California, USA.
Sebastian AdlawanDepartment of Surgery, University of California San Diego, La Jolla, California, USA.
Brian P EliceiriDepartment of Surgery, University of California San Diego, La Jolla, California, USA; Department of Dermatology, University of California San Diego, La Jolla, California, USA. Electronic address: beliceiri@health.ucsd.edu.

Funding

UC San Diego Clinical and Translational Research InstituteKL2TR001444 · NCATS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI DEPP, COLIN A. · 2015 to 2024
$15.4M
Tissue repair, extracellular vesicular biogenesis, and the control of immune responsesR01GM140137 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI ELICEIRI, BRIAN P · 2020 to 2023
$1.8M
Mechanisms of extracellular vesicle biogenesis that regulate wound healingR35GM149245 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI Brian P Eliceiri · 2023 to 2026
$1.7M
The role of the ECRG4 in Host Defense of Staph Aureus InfectionK08AR077734 · NIAMS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI DORSCHNER, ROBERT A · 2020 to 2024
$775k
Acquisition of an Orbitrap Elite with ETDS10OD021724 · OD · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI KOMIVES, ELIZABETH A. · 2016 to 2016
$600k
NCATS NIH HHS KL2 TR001444NIAMS NIH HHS K08 AR077734NIGMS NIH HHS R01 GM140137NIGMS NIH HHS R35 GM149245NIH HHS S10 OD021724
6 · The paper itself

Abstract

Normal cutaneous wound healing is a multicellular process that involves the release of small extracellular vesicles (sEVs) that coordinate intercellular communication by delivery of sEV payloads to recipient cells. We have recently shown how the proreparative activity of inflammatory cell sEVs, especially macrophage and neutrophil-derived sEVs, in the wound bed is dysregulated in impaired wound healing. In this study, we show that loss of Rab27A, a small GTPase that has a regulatory function in sEV secretion, reduces the release of neutrophil and macrophage-derived sEVs. Proteomic profiling of sEVs identified Rab27A-dependent protein payloads relevant in neutrophil activation, such as CD44, leukotriene A4 hydrolase, complement C5, and neutrophilic granule protein. We defined the activity of sEVs derived from Rab27A-knockout donors by demonstrating a delayed neutrophil recruitment in the initial injury response. The dysfunction of Rab27-knockout sEVs was linked to an increase in the release of CCL4, consistent with the sustained inflammation observed in vivo and reduced neutrophil migration in vitro. Together, these findings show how a specific regulator of sEV biogenesis can direct protein payloads and activities that mediate neutrophil trafficking in wound healing.

Indexed as

Extracellular VesiclesNeutrophil InfiltrationNeutrophilsSkinWound HealingAnimalsCells, CulturedHumansMacrophagesMiceMice, Inbred C57BLMice, KnockoutProteomicsrab27 GTP-Binding ProteinsRAB27A protein, humanRab27a protein, mouserab27 GTP-Binding ProteinsExosomeExtracellular vesiclesNeutrophil recruitmentRab27AWound healing

Identifiers

PMID40914410
PMCPMC12682223

What Socratic holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.