Evidence map›Paper›PMID 40915528›Full record

ArticleDevelopmental biology2025

CXXC Finger Protein 1 drives BMP signaling and progenitor cell differentiation during limb development.

Emanuele Pignatti, Lijie Jiang, Manasvi S Shah, Anish Karpurapu, Ji Miao, Yangyang Liu, Mesut Berber, Roshini Kalagara, Aisling E Butler, David G Skalnik and 3 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Developmental biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Emanuele PignattiDivision of Endocrinology, Boston Children's Hospital, Boston, MA, 02115, USA; Department of Pediatrics, Harvard Medical School, Boston, MA, 02115, USA.
Lijie JiangDivision of Endocrinology, Boston Children's Hospital, Boston, MA, 02115, USA.
Manasvi S ShahDivision of Endocrinology, Boston Children's Hospital, Boston, MA, 02115, USA; Department of Pediatrics, Harvard Medical School, Boston, MA, 02115, USA.
Anish KarpurapuDivision of Endocrinology, Boston Children's Hospital, Boston, MA, 02115, USA; Harvard Stem Cell Institute, 7 Divinity Ave, Cambridge, MA, 02138, USA.
Ji MiaoDivision of Endocrinology, Boston Children's Hospital, Boston, MA, 02115, USA; Department of Pediatrics, Harvard Medical School, Boston, MA, 02115, USA.
Yangyang LiuDivision of Endocrinology, Boston Children's Hospital, Boston, MA, 02115, USA.
Mesut BerberDivision of Endocrinology, Boston Children's Hospital, Boston, MA, 02115, USA; Department of Pediatrics, Harvard Medical School, Boston, MA, 02115, USA.
Roshini KalagaraDivision of Endocrinology, Boston Children's Hospital, Boston, MA, 02115, USA; Harvard Stem Cell Institute, 7 Divinity Ave, Cambridge, MA, 02138, USA.
Aisling E ButlerDivision of Endocrinology, Boston Children's Hospital, Boston, MA, 02115, USA.
David G SkalnikDepartment of Biology, IUPUI School of Science, Indianapolis, IN, 46202, USA.
Vicki RosenHarvard Stem Cell Institute, 7 Divinity Ave, Cambridge, MA, 02138, USA; Department of Developmental Biology, Harvard School of Dental Medicine, Boston, MA, 02115, USA.
David T BreaultDivision of Endocrinology, Boston Children's Hospital, Boston, MA, 02115, USA; Department of Pediatrics, Harvard Medical School, Boston, MA, 02115, USA; Harvard Stem Cell Institute, 7 Divinity Ave, Cambridge, MA, 02138, USA.
Diana L CarloneDivision of Endocrinology, Boston Children's Hospital, Boston, MA, 02115, USA; Department of Pediatrics, Harvard Medical School, Boston, MA, 02115, USA; Harvard Stem Cell Institute, 7 Divinity Ave, Cambridge, MA, 02138, USA. Electronic address: diana.carlone@childrens.harvard.edu.

Funding

Translational Imaging and Phenotyping CoreP30AR075042 · NIAMS · MASSACHUSETTS GENERAL HOSPITAL · PI Marie Demay · 2019 to 2026
$7.4M
Skeletal Phenotyping CoreP30AR066261 · NIAMS · MASSACHUSETTS GENERAL HOSPITAL · PI KRONENBERG, HENRY M. · 2014 to 2018
$3.5M
Cfp1 Action in Cartilage DevelopmentR01AR074982 · NIAMS · BOSTON CHILDREN'S HOSPITAL · PI CARLONE, DIANA LYNN · 2020 to 2024
$2.2M
Transitional Growth-Associated Skeletal Stem CellsR03AG054723 · NIA · BOSTON CHILDREN'S HOSPITAL · PI CARLONE, DIANA LYNN · 2017 to 2018
$354k
NIAMS NIH HHS P30 AR066261NIAMS NIH HHS P30 AR075042NIAMS NIH HHS R01 AR074982NIA NIH HHS R03 AG054723
6 · The paper itself

Abstract

The mechanisms mediating endochondral bone formation remain incompletely understood. Here, we show that CXXC Finger Protein 1 (CFP1) is required for the onset of chondrogenesis during forelimb development. CFP1-deficient mesenchymal progenitor cells (LMPs) retain an immature molecular signature with elevated FGF and SHH signaling and repressed BMP signaling, in part, due to (1) reduced expression of type I BMP receptors, (2) reduced Smad1 protein levels and (3) an altered extracellular niche. Moreover, the addition of exogenous BMP ligand or antagonism of heparan sulfate restores LMP differentiation toward a chondrogenic fate and enhances BMP signaling, suggesting a defect in BMP ligand bioavailability mediates the CFP1-deficient LMP phenotype. Together, these findings define CFP1 as a gatekeeper between the undifferentiated and differentiated state of LMPs during endochondral bone formation and as a physiological regulator of BMP signaling. CLASSIFICATION: Biological Sciences.

Indexed as

Bone Morphogenetic ProteinsCell DifferentiationExtremitiesMesenchymal Stem CellsSignal TransductionAnimalsChondrogenesisGene Expression Regulation, DevelopmentalHedgehog ProteinsMiceOsteogenesisSmad1 ProteinBone Morphogenetic ProteinsHedgehog ProteinsSmad1 ProteinBMP signalingChondrogenesisDifferentiationEpigenetics

Identifiers

PMID40915528
PMCPMC12481203

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.