Evidence map›Paper›PMID 40916017›Full record

ArticleAnnals of surgical oncology2025

Single-Cell RNA Sequencing Reveals Potential Mechanism of RUNX3 Reshaping Tumor Microenvironment in Non-small-cell Lung Cancer.

Weijin Xiao, Jianqing Zheng, Yujie Deng, Bifen Huang, Weibin Liu, Xuejin Zheng, Kaijia Zhou, Weimin Fang, Xiaohui Chen

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Annals of surgical oncology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Weijin Xiao *Department of Pathology, Clinical Oncology School of Fujian Medical University, Fujian Cancer Hospital, Fuzhou, China.
Jianqing Zheng *Department of Radiation Oncology, The Second Affiliated Hospital of Fujian Medical University, Quanzhou, China.
Yujie Deng *Department of Medical Oncology, The First Affiliated Hospital of Fujian Medical University, Fuzhou, China.
Bifen HuangDepartment of Obstetrics and Gynecology, People's Hospital Affiliated of Quanzhou Medical College, Quanzhou, China.
Weibin LiuDepartment of Thoracic Surgery, Clinical Oncology School of Fujian Medical University, Fujian Cancer Hospital, Fuzhou, Fujian Province, China.
Xuejin ZhengInterdisciplinary Institute of Medical Engineering of Fuzhou University, Fuzhou, China.
Kaijia ZhouInterdisciplinary Institute of Medical Engineering of Fuzhou University, Fuzhou, China.
Weimin FangDepartment of Thoracic Surgery, Clinical Oncology School of Fujian Medical University, Fujian Cancer Hospital, Fuzhou, Fujian Province, China.
Xiaohui ChenDepartment of Thoracic Surgery, Clinical Oncology School of Fujian Medical University, Fujian Cancer Hospital, Fuzhou, Fujian Province, China. josephcxh@fjmu.edu.cn.

Funding

Fujian Provincial Academic and Research Program for Young and Middle-age Teachers JAT220083Fujian Provincial Natural and Scientific Foundation 2023J011255Joint Funds for the Innovation of Science and Technology, Fujian Province 2020Y9036Science and technology projects of Quanzhou city 2023NS010The Natural Science Foundation of Fujian Province 2024J01671
6 · The paper itself

Abstract

backgroundRUNX3 acts as a tumor suppressor gene in non-small-cell lung cancer (NSCLC), yet its specific biological mechanism is still unclear. This study aimed to uncover tumor microenvironment (TME) changes in NSCLC with varying RUNX3 expression statuses through single-cell RNA sequencing. PATIENTS AND

methodsIn total, seven patients with NSCLC with detailed pathological data were involved, with three both paracancerous and cancerous tissue samples. After sequencing, the "Seurat" package was used to analyze differentially expressed genes, annotate cell clusters with marker genes, and compare cell proportion differences at different RUNX3 expression levels. Observed-over-expected cell number ratios (R

resultsImmunohistochemical staining of RUNX3 categorized three patients into the RUNX3-negative group (RUNX3_Neg) and four into the RUNX3 positive group (RUNX3_Pos). All cells were classified into 13 types based on marker genes. R

conclusionsThrough single-cell sequencing, our study found that RUNX3 expression status is closely related to NSCLC TME. Mononuclear phagocytes may be an important target cell population for RUNX3 to change TME.

Indexed as

Biomarkers, TumorCarcinoma, Non-Small-Cell LungCore Binding Factor Alpha 3 SubunitLung NeoplasmsSingle-Cell AnalysisTumor MicroenvironmentAgedFemaleFollow-Up StudiesGene Expression Regulation, NeoplasticHumansMaleMiddle AgedPrognosisSequence Analysis, RNABiomarkers, TumorCore Binding Factor Alpha 3 SubunitRunx3 protein, humanNon-small-cell lung cancerRUNX3Single-cell RNA sequencingTumor-associated macrophageTumor microenvironment

Identifiers

PMID40916017
PMCPMC12589342

What Socratic holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.