Evidence mapPaperPMID 40923494Full record

ArticleFEBS open bio2026

Electrical pulse stimulation reflecting the episodic nature of real-life exercise modulates metabolic and secretory profile of primary human myotubes.

Klára Gabrišová, Tímea Kurdiová, Daria Barkova, Natália Pálešová, Jana Babulicová, Silvia Tyčiaková, Marta Novotová, Mária Balážová, Miroslav Sabo, Václav Pustka and 2 more

Abstract read
In one paragraph

Article in FEBS open bio, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Enhancing Maturation of Human Neuromuscular Organoids via Electrical Stimulation.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Klára GabrišováDepartment of Metabolic Disease Research, Institute of Experimental Endocrinology, Biomedical Research Center, Slovak Academy of Sciences, Bratislava, Slovakia.ORCID https://orcid.org/0009-0009-4469-1169
Tímea KurdiováDepartment of Metabolic Disease Research, Institute of Experimental Endocrinology, Biomedical Research Center, Slovak Academy of Sciences, Bratislava, Slovakia.ORCID https://orcid.org/0000-0003-1686-3647
Daria BarkovaDepartment of Metabolic Disease Research, Institute of Experimental Endocrinology, Biomedical Research Center, Slovak Academy of Sciences, Bratislava, Slovakia.ORCID https://orcid.org/0000-0001-9807-6916
Natália PálešováDepartment of Metabolic Disease Research, Institute of Experimental Endocrinology, Biomedical Research Center, Slovak Academy of Sciences, Bratislava, Slovakia.ORCID https://orcid.org/0009-0008-8514-4068
Jana BabulicováDepartment of Metabolic Disease Research, Institute of Experimental Endocrinology, Biomedical Research Center, Slovak Academy of Sciences, Bratislava, Slovakia.ORCID https://orcid.org/0009-0000-3212-5738
Silvia TyčiakováDepartment of Molecular Oncology, Cancer Research Institute, Biomedical Research Center, Slovak Academy of Sciences, Bratislava, Slovakia.ORCID https://orcid.org/0000-0003-2919-0410
Marta NovotováDepartment of Cellular Cardiology, Institute of Experimental Endocrinology, Biomedical Research Center, Slovak Academy of Sciences, Bratislava, Slovakia.ORCID https://orcid.org/0000-0002-1514-1995
Mária BalážováDepartment of Membrane Biochemistry, Institute of Animal Biochemistry and Genetics, Centre of Biosciences, Slovak Academy of Sciences, Bratislava, Slovakia.ORCID https://orcid.org/0000-0001-8113-6855
Miroslav SaboDepartment of Bioinformatics, Institute of Experimental Endocrinology, Biomedical Research Center, Slovak Academy of Sciences, Bratislava, Slovakia.ORCID https://orcid.org/0009-0009-2112-2502
Václav PustkaCentral European Institute of Technology, Masaryk University, Brno, Czech Republic.ORCID https://orcid.org/0009-0005-9857-3601
Jozef UkropecDepartment of Metabolic Disease Research, Institute of Experimental Endocrinology, Biomedical Research Center, Slovak Academy of Sciences, Bratislava, Slovakia.ORCID https://orcid.org/0000-0001-8401-6621
Barbara UkropcováDepartment of Metabolic Disease Research, Institute of Experimental Endocrinology, Biomedical Research Center, Slovak Academy of Sciences, Bratislava, Slovakia.ORCID https://orcid.org/0000-0002-3309-7713

Funding

Grant Agency of the Slovak Academy of Sciences VEGA 2/0076/22Grant Agency of the Slovak Academy of Sciences VEGA 2/0144/23MEYS CR e-INFRA CZ ID:90254MEYS CR LM2023042Ministry of Health of the Czech Republic NU23-01-00509Slovak Research and Development Agency APVV 20-0466Slovak Research and Development Agency APVV 23-0604
6 · The paper itself

Abstract

Electrical pulse stimulation (EPS) represents a useful tool to study exercise-related adaptations of muscle cells in vitro. Here, we examine the metabolic and secretory response of primary human muscle cells from metabolically healthy individuals to the EPS protocol reflecting the episodic nature of real-life exercise training. This intermittent EPS protocol alternates high-frequency stimulation periods with low-frequency resting periods. Continuous EPS was used as a comparator. Radiometric assessment of glucose and fatty acid metabolism was complemented by examination of mitochondrial OxPHOS proteins, fiber-type markers, and the release of selected myokines and extracellular vesicles into the media. Both EPS protocols facilitated glycogen synthesis and incomplete fatty acid oxidation (intermediary metabolites accumulation), while complete glucose and fatty acid oxidation (CO

Indexed as

Electric StimulationExerciseMuscle Fibers, SkeletalAdultCells, CulturedFatty AcidsGlucoseHumansMaleMuscle, SkeletalFatty AcidsGlucoseelectrical pulse stimulationextracellular vesiclesglucose and fatty acid metabolismgrowth differentiation factor 11human primary muscle cell culturemyokines

Identifiers

PMID40923494
PMCPMC12767763

What Socratic holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.