Evidence map›Paper›PMID 40927176›Full record

ArticleFrontiers in veterinary science2025

Development of a reverse genetics system for West Nile virus (Kunjin type).

Zhen Wu, Tao Hu, Zhou Zhou, Yu He, Tao Wang, Mingshu Wang, Renyong Jia, Dekang Zhu, Mafeng Liu, Xinxin Zhao and 9 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Frontiers in veterinary science, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Application of Orthoflavivirus Pseudovirus Technology in Antiviral Research.International journal of molecular sciences · 2026
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

19 authors.

Zhen Wu *Institute of Veterinary Medicine and Immunology, Sichuan Agricultural University, Chengdu, Sichuan, China.
Tao Hu *Institute of Veterinary Medicine and Immunology, Sichuan Agricultural University, Chengdu, Sichuan, China.
Zhou Zhou *Institute of Veterinary Medicine and Immunology, Sichuan Agricultural University, Chengdu, Sichuan, China.
Yu HeInstitute of Veterinary Medicine and Immunology, Sichuan Agricultural University, Chengdu, Sichuan, China.
Tao WangInstitute of Veterinary Medicine and Immunology, Sichuan Agricultural University, Chengdu, Sichuan, China.
Mingshu WangInstitute of Veterinary Medicine and Immunology, Sichuan Agricultural University, Chengdu, Sichuan, China.
Renyong JiaInstitute of Veterinary Medicine and Immunology, Sichuan Agricultural University, Chengdu, Sichuan, China.
Dekang ZhuInstitute of Veterinary Medicine and Immunology, Sichuan Agricultural University, Chengdu, Sichuan, China.
Mafeng LiuInstitute of Veterinary Medicine and Immunology, Sichuan Agricultural University, Chengdu, Sichuan, China.
Xinxin ZhaoInstitute of Veterinary Medicine and Immunology, Sichuan Agricultural University, Chengdu, Sichuan, China.
Qiao YangInstitute of Veterinary Medicine and Immunology, Sichuan Agricultural University, Chengdu, Sichuan, China.
Ying WuInstitute of Veterinary Medicine and Immunology, Sichuan Agricultural University, Chengdu, Sichuan, China.
Shaqiu ZhangInstitute of Veterinary Medicine and Immunology, Sichuan Agricultural University, Chengdu, Sichuan, China.
Juan HuangInstitute of Veterinary Medicine and Immunology, Sichuan Agricultural University, Chengdu, Sichuan, China.
Xumin OuInstitute of Veterinary Medicine and Immunology, Sichuan Agricultural University, Chengdu, Sichuan, China.
Di SunInstitute of Veterinary Medicine and Immunology, Sichuan Agricultural University, Chengdu, Sichuan, China.
Bin TianInstitute of Veterinary Medicine and Immunology, Sichuan Agricultural University, Chengdu, Sichuan, China.
Anchun ChengInstitute of Veterinary Medicine and Immunology, Sichuan Agricultural University, Chengdu, Sichuan, China.
Shun ChenInstitute of Veterinary Medicine and Immunology, Sichuan Agricultural University, Chengdu, Sichuan, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Kunjin virus (KUNV), a naturally attenuated strain of West Nile virus (WNV), shares similar transmission modes and hosts-primarily mosquitoes, birds, and horses. Globally, reverse genetics is the principal methodology for characterizing the molecular etiology of flaviviruses. In this study, cytomegalovirus (CMV) promoter-driven KUNV reporter replicons were engineered to incorporate three distinct reporter genes: Nanoluc, oxGFP, and mCherry. These replicons demonstrated successful translation and replication in mammalian (BHK-21), avian (DEF), and avian hepatic (LMH) cell lines. Additionally, an

Indexed as

Kunjin viruspackaging systemreporter repliconreporter virusreverse genetics system

Identifiers

PMID40927176
PMCPMC12414792

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.