Evidence map›Paper›PMID 40928544›Full record

ArticleFunctional & integrative genomics2025

Key copper homeostasis genes and inflammatory mechanisms in ischemic stroke: A bioinformatics and experimental study.

Ting Shi, Zhifeng Wang, Jiao Yang, Pengfen He, Daman Tian, Junfeng Lan, Shuangfeng Xu, Aiming Yang, Liwei Xing, Yujiang Xi and 1 more

Abstract read
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In one paragraph

Article in Functional & integrative genomics, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
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0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

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0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Ting Shi *The First Clinical Medical College, Yunnan University of Chinese Medicine, Kunming, China.
Zhifeng Wang *The First Clinical Medical College, Yunnan University of Chinese Medicine, Kunming, China.
Jiao Yang *College of Acupuncture and Massage, Yunnan University of Chinese Medicine, Kunming, China.
Pengfen HeThe First Clinical Medical College, Yunnan University of Chinese Medicine, Kunming, China.
Daman TianThe First Clinical Medical College, Yunnan University of Chinese Medicine, Kunming, China.
Junfeng LanThe First Clinical Medical College, Yunnan University of Chinese Medicine, Kunming, China.
Shuangfeng XuThe First Clinical Medical College, Yunnan University of Chinese Medicine, Kunming, China.
Aiming YangYunnan Provincial Hospital of Traditional Chinese Medicine, Kunming, China.
Liwei XingThe First Clinical Medical College, Yunnan University of Chinese Medicine, Kunming, China. 1791536722@qq.com.
Yujiang XiThe First Clinical Medical College, Yunnan University of Chinese Medicine, Kunming, China. 342133033@qq.com.
Jian WangThe First Clinical Medical College, Yunnan University of Chinese Medicine, Kunming, China. laohaodiao@126.com.

Funding

Basic Research Program Project of Yunnan Provincial Science and Technology Department 202101AZ070001-255General Project of Yunnan Applied Basic Research Program No. 202201AT070214)two types of talent projects in Yunnan Province No. 202205AD160024Yunnan Provincial Science and Technology Department-Applied Basic Research Joint Special Funds of Chinese Medicine Nos. 202001AZ070001-020, 202301AZ070001-016
6 · The paper itself

Abstract

Ischemic stroke (IS) has high morbidity/mortality with limited treatments. This study screened core copper homeostasis-related genes in IS and validated their function as precise intervention targets. Human IS gene chip data were retrieved from GEO, and copper homeostasis genes from multiple databases. After data correction/normalization, IS differentially expressed genes (DEGs) were identified and intersected with copper genes. DAVID for GO/KEGG enrichment, STRING for PPI network, CytoHubba for key genes, and ROC curves for diagnostic value. MCAO rat models were established; after 14-day rearing, protein expression was validated via relevant assays. 1,425 IS DEGs and 2,610 copper genes intersected to 235 genes, enriched in inflammatory response, innate immunity, and TNF/NOD-like/Toll-like receptor pathways. Key genes HIF-1α, TNF, TLR4, IL-1β had IS diagnostic AUC 0.70-0.80. MCAO rats showed worse neurological deficits, larger infarcts, neuronal damage, brain copper accumulation, and upregulated key genes linked to neuroinflammation, BBB disruption, and neuronal injury. Bioinformatics and animal studies revealed copper homeostasis imbalance-related genes mediate inflammation, oxidative stress, and mitochondrial regulation, supporting the "copper accumulation-inflammation-oxidative stress" cascade. These genes may be novel IS targets. Further study on dynamic expression across ischemic time windows/brain regions and interactions with other cell death modes is needed.

Indexed as

CopperInflammationIschemic StrokeAnimalsComputational BiologyCytokinesDisease Models, AnimalHomeostasisHumansHypoxia-Inducible Factor 1, alpha SubunitMaleNF-kappaB-Inducing KinaseOligonucleotide Array Sequence AnalysisOxidative StressProtein Interaction MapsRatsCopperCytokinesHif1a protein, ratHypoxia-Inducible Factor 1, alpha SubunitNF-kappaB-Inducing KinaseTlr4 protein, ratToll-Like Receptor 4Animal experiments verificationBioinformatics analysisCopper homeostasisIschemic strokeKey genes

Identifiers

PMID40928544

What Socratic holds

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.