Evidence map›Paper›PMID 40932298›Full record

ArticleDiabetes2025

Serum Metabolomics Reveals Potential Differences in Gut Microbiota-Associated Metabolites in Twins Discordant for Type 1 Diabetes.

Elizabeth R Flammer, Michael W Christopher, Esabella R Powers, Hali Broncucia, Andrea K Steck, Stephen E Gitelman, Timothy J Garrett, Heba M Ismail

Abstract readTwin Study
In one paragraph

Article in Diabetes, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

8 authors.

Elizabeth R FlammerDepartment of Chemistry, College of Liberal Arts and Sciences, University of Florida, Gainesville, FL.
Michael W ChristopherDepartment of Chemistry, College of Liberal Arts and Sciences, University of Florida, Gainesville, FL.
Esabella R PowersDepartment of Chemistry, College of Liberal Arts and Sciences, University of Florida, Gainesville, FL.
Hali BroncuciaBarbara Davis Center for Childhood Diabetes, School of Medicine, University of Colorado, Aurora, CO.
Andrea K SteckBarbara Davis Center for Childhood Diabetes, School of Medicine, University of Colorado, Aurora, CO.
Stephen E GitelmanDepartment of Pediatrics, University of California San Francisco, San Francisco, CA.
Timothy J GarrettDepartment of Chemistry, College of Liberal Arts and Sciences, University of Florida, Gainesville, FL.ORCID 0000-0003-1623-009X
Heba M IsmailDepartment of Pediatrics, School of Medicine, Indiana University, Indianapolis, IN.ORCID 0000-0003-0102-0030

Funding

Translation CoreP30DK097512 · NIDDK · INDIANA UNIVERSITY INDIANAPOLIS · PI JEFFREY S ELMENDORF · 2015 to 2026
$17.4M
The Gut Microbiome in Lean and Obese Youth with Type 1 Diabetes and Novel Mechanism of Action of MetforminK23DK129799 · NIDDK · INDIANA UNIVERSITY INDIANAPOLIS · PI ISMAIL, HEBATULLAH · 2022 to 2025
$730k
National Institute of Diabetes and Digestive and Kidney Diseases of the National Institutes of Health DK-129799NIDDK NIH HHS K23 DK129799NIDDK NIH HHS P30 DK097512
6 · The paper itself

Abstract

We investigated serum metabolites in monozygotic (MZ) and dizygotic (DZ) twins discordant for type 1 diabetes (T1D) to explore potential environmental factors, with a focus on differences in gut microbiota-associated metabolites that may influence T1D. Serum samples from 39 twins discordant for T1D were analyzed using a semi-targeted metabolomics approach via liquid chromatography-high-resolution tandem mass spectrometry. Statistical analyses identified significant metabolites (P < 0.1) within three groups: all twins (combined group [All]), MZ twins, and DZ twins. Thirteen metabolites exhibited significant differences between individuals with T1D and those without T1D. Across all groups, 3-indoxyl sulfate and 5-hydroxyindole were significantly reduced in individuals with T1D. Carnitine was reduced, and threonine, muramic acid, and 2-oxobutyric acid were significantly elevated in both All and MZ groups. Allantoin was significantly reduced and 3-methylhistidine was significantly elevated in All and DZ groups. These findings suggest metabolite dysregulation associated with gut dysbiosis was observed. However, further validation of our findings in a larger cohort is needed. ARTICLE HIGHLIGHTS: We believed this cohort of twins discordant for type 1 diabetes (T1D) would allow for control over genetic variability to examine environmental factors. We aimed to identify differences in microbial and microbiota-associated metabolites in twins discordant for T1D to examine the effect of the gut microbiome on T1D. Thirteen metabolites were identified as significantly different. Our results show dysregulation of several microbial metabolites in twin pairs, suggesting the role of the gut microbiome in T1D pathogenesis.

Indexed as

Diabetes Mellitus, Type 1Gastrointestinal MicrobiomeAdolescentAdultChildDysbiosisFemaleHumansMaleMetabolomeMetabolomicsTwins, DizygoticTwins, MonozygoticYoung Adult

Identifiers

PMID40932298
PMCPMC12585161

What Socratic holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.