Comprehensive Analysis of the Microbiome and Metabolome: Unveiling Interactions Between Intestinal Flora and Metabolites in Schizophrenia.

Background: Schizophrenia (SZ) is a complex, multifactorial neurodevelopmental disorder characterized as a severe mental illness. Despite extensive research, its etiology and pathogenesis remain largely elusive. Recent studies suggest that the intestinal microbiota and its metabolites may play critical roles in the onset and regulation of SZ. This study aims to examine the characteristics of the intestinal microbiota and metabolomics in patients with SZ and to explore their correlation with clinical symptoms. Methods: This study involved thirty-five patients diagnosed with schizophrenia (SZ group) and thirty healthy control participants (HC group). Fecal samples were collected and analyzed using microbiome (16S rRNA gene sequencing) and metabolome (UPLC-MS) techniques. Bioinformatics and statistical analyses were conducted to interpret the data. Results: Age, sex, and BMI were comparable between the SZ and HC groups. Microbial diversity sequencing identified 837 operational taxonomic units (OTUs) across both groups. Although alpha diversity metrics showed no significant difference, beta diversity metrics revealed notable disparities. At the genus level, differences were observed in nine bacterial groups: Conclusion: The study reveals significant abnormalities in the intestinal flora and metabolites of SZ patients, correlating with clinical severity. These findings offer new insights into the interactions between the gut microbiome and metabolites, potentially contributing to the understanding of the pathophysiology of schizophrenia.