Protocol for noninvasive imaging of hematopoietic reconstitution in live mice using Akaluc bioluminescence.

Takao Yogo, Satoshi Yamazaki

Abstract read

In one paragraph

Article in STAR protocols, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Takao YogoDivision of Cell Regulation, Center for Experimental Medicine and Systems Biology, The Institute of Medical Science, The University of Tokyo, Tokyo, Japan. Electronic address: takayogo0430@g.ecc.u-tokyo.ac.jp.

Satoshi YamazakiDivision of Cell Regulation, Center for Experimental Medicine and Systems Biology, The Institute of Medical Science, The University of Tokyo, Tokyo, Japan; Division of Cell Engineering, Center for Stem Cell Biology and Regenerative Medicine, The Institute of Medical Science, The University of Tokyo, Tokyo, Japan; Laboratory for Stem Cell Therapy, Faculty of Medicine, Tsukuba University, Ibaraki, Japan. Electronic address: y-sato4@g.ecc.u-tokyo.ac.jp.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Here, we present a protocol for noninvasive, spatiotemporal visualization of hematopoietic reconstitution in live mice using Akaluc-based bioluminescence imaging (AkaBLI). We describe steps for Akaluc gene transduction into murine hematopoietic stem cells (HSCs), ex vivo expansion of hematopoietic stem and progenitor cells, and longitudinal imaging post transplantation. Representative results illustrate the spatiotemporal pattern of hematopoietic recovery in the spleen and bone marrow. This protocol enables quantitative in vivo tracking of hematopoietic reconstitution dynamics at whole-body resolution following lethal irradiation. For complete details on the use and execution of this protocol, please refer to Yogo et al.

Indexed as

HematopoiesisHematopoietic Stem CellsLuminescent MeasurementsAnimalsHematopoietic Stem Cell TransplantationMiceCell BiologyCell cultureCell DifferentiationStem Cells

Identifiers

PMID40934075

DOI10.1016/j.xpro.2025.104072

PMCPMC12491520

What Socratic holds

Textmetadata

LicenceCC BY-NC-ND

Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.