Evidence map›Paper›PMID 40934076›Full record

ArticleSTAR protocols2025

Protocol for screening inhibitors of the fungal sphingolipid-metabolizing enzyme ceramide synthase.

Andy Zhong, Deveney Dasilva, Nivea Pereira de Sa, Jeehyun Karen You, Maurizio Del Poeta

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in STAR protocols, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Andy ZhongDepartment of Microbiology and Immunology, Stony Brook University, Stony Brook, NY 11794, USA.
Deveney DasilvaDepartment of Microbiology and Immunology, Stony Brook University, Stony Brook, NY 11794, USA.
Nivea Pereira de SaDepartment of Microbiology and Immunology, Stony Brook University, Stony Brook, NY 11794, USA.
Jeehyun Karen YouDepartment of Microbiology and Immunology, Stony Brook University, Stony Brook, NY 11794, USA.
Maurizio Del PoetaDepartment of Microbiology and Immunology, Stony Brook University, Stony Brook, NY 11794, USA; Veterans Affairs Medical Center, Northport, NY, USA; Division of Infectious Diseases, School of Medicine, Stony Brook University, Stony Brook, NY, USA; MicroRid Technologies Inc., Dix Hills, NY, USA. Electronic address: maurizio.delpoeta@stonybrook.edu.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Fungal sphingolipids have emerged as potent antifungal targets based on cumulative evidence demonstrating that fungal sphingolipid metabolism is essential to the virulence of pathogenic fungi. Here, we present a protocol to screen for inhibitors of the sphingolipid-metabolizing enzyme ceramide synthase. We describe steps for harvesting microsomes from Saccharomyces cerevisiae, performing in vitro reactions, and performing lipid extractions. We then detail the use of a high-performance liquid chromatography (HPLC)-based fluorometric assay to screen large chemical libraries for ceramide synthase inhibitors. For complete details on the use and execution of this protocol, please refer to Dasilva et al.

Indexed as

Antifungal AgentsEnzyme InhibitorsOxidoreductasesSaccharomyces cerevisiaeSphingolipidsChromatography, High Pressure LiquidDrug Evaluation, PreclinicalMicrosomesAntifungal Agentsdihydroceramide desaturaseEnzyme InhibitorsOxidoreductasesSphingolipidsCell-based AssaysHigh-Throughput ScreeningMicrobiology

Identifiers

PMID40934076
PMCPMC12491512

What Socratic holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.