Green Synthesized Silver Nanoparticles from Meyna Laxiflora: Binding with HSA and Anti-biofilm Potential.

Nanomaterials offer tremendous potential for altering the therapeutic and pharmacokinetic properties of molecular drugs. Herein, we introduce a rapid method for synthesizing silver nanoparticles using Meyna laxiflora leaf extracts and assessing their biological activity. In characterization, UV-Visible spectroscopy confirms the formation of AgNPs. Transmission electron microscopy confirms that the size of Meyna laxiflora-AgNPs (ML-AgNPs) is 14.7 nm. The nanoparticles were uniformly sized and stabilized by phytochemical functional groups (-OH, -CO,-COOH, C-O-C) as confirmed through physicochemical characterization. After the characterization, the binding studies with HSA were analyzed via fluorescence spectroscopy, TCSPC lifetime measurement, circular dichroism spectroscopy, and Isothermal Titration Calorimetry (ITC). Binding studies revealed strong interactions with human serum albumin (HSA), involving both endothermic and exothermic processes and the binding is static in nature and have minimal conformational changes in HSA. ML-AgNPs show anticancer activities towards cervical and lung cancer cell lines. The scavenging activity of the ML-AgNPs indicates it can act as a good antioxidant. The anti-biofilm potential of the ML-AgNPs shows, it can act as a promising anti-biofilm agent. Thus, we conclude that ML-AgNPs could be used as an anticancer, antioxidant, and anti-biofilm agent and hold a potential candidate for nanomedicine.