ArticleSTAR protocols2025
Protocol for monitoring mRNA translation and degradation in human cell-free lysates.
Article in STAR protocols, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
2 citing papers in PubMed.
- Article
- A human cell-free translation screen identifies the NT-2 mycotoxin as a ribosomal inhibitor that binds the peptidyl transferase center.Communications biology · 2026Article
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Authors and funding
2 authors.
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Abstract
Cell-free translation is a powerful tool for studying protein synthesis. Here, we present a protocol for monitoring mRNA translation and degradation using a human cell-free translation system. The protocol includes instructions for preparing translation-competent lysates via dual centrifugation, generating and capping reporter mRNAs compatible with luciferase assays, performing cell-free translation reactions, and assessing mRNA stability by northern blotting. The protocol supports comparative analysis of translation efficiency and mRNA decay for various 5' UTRs and viral RNA elements. For complete details on the use and execution of this protocol, please refer to Gurzeler et al.
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Registered trials
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