Evidence map›Paper›PMID 40938751›Full record

ArticleSTAR protocols2025

Protocol to generate stem cell-derived alpha cells in 3D suspension culture.

Tiana Salomon, Kyle R Knofczynski, Swikriti Shrestha, Quinn P Peterson

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in STAR protocols, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Predictive control of human pancreatic cell fate using a digital model ofbioRxiv : the preprint server for biology · 2026
    Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Tiana SalomonMayo Clinic Graduate School of Biomedical Sciences, Mayo Clinic, Rochester, MN 55902, USA; Islet Engineering and Replacement Laboratory, Mayo Clinic, Rochester, MN 55902, USA.
Kyle R KnofczynskiMayo Clinic Graduate School of Biomedical Sciences, Mayo Clinic, Rochester, MN 55902, USA; Islet Engineering and Replacement Laboratory, Mayo Clinic, Rochester, MN 55902, USA.
Swikriti ShresthaMayo Clinic Graduate School of Biomedical Sciences, Mayo Clinic, Rochester, MN 55902, USA; Islet Engineering and Replacement Laboratory, Mayo Clinic, Rochester, MN 55902, USA.
Quinn P PetersonIslet Engineering and Replacement Laboratory, Mayo Clinic, Rochester, MN 55902, USA; Department of Physiology and Biomedical Engineering, Mayo Clinic, Rochester, MN 55902, USA. Electronic address: peterson.quinn@mayo.edu.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Pancreatic alpha cells represent a key islet cell type that acts in tandem with pancreatic beta cells to regulate blood glucose levels. Here, we present a protocol to generate stem cell-derived alpha (SC-α) cells using chemically defined small-molecule and growth factor induction in a scalable 3D suspension culture. We describe steps for embryonic stem cell culture and SC-α differentiation. We then detail procedures for quality control and the assessment of SC-α cell function. For complete details on the use and execution of this protocol, please refer to Peterson et al.

Indexed as

Cell Culture TechniquesCell Culture Techniques, Three DimensionalGlucagon-Secreting CellsAnimalsCell DifferentiationCells, CulturedEmbryonic Stem CellsMiceCell BiologyCell cultureCell DifferentiationDevelopmental biologyOrganoidsStem Cells

Identifiers

PMID40938751
PMCPMC12491158

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.