Evidence map›Paper›PMID 40939153›Full record

ArticleChembiochem : a European journal of chemical biology2025

Two-Color Timestamping of Gene Expression with a Chemigenetic Reporter System.

Henriette Lämmermann, Jade Nguyen, Juan F Tamez-Fernández, Fabien Kuttler, Julien Bortoli Chapalay, Marc Chambon, Gerardo Turcatti, Pablo Rivera-Fuentes

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Chembiochem : a European journal of chemical biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Two-Color Timestamping of Gene Expression with a Chemigenetic Reporter System.Chembiochem : a European journal of chemical biology · 2025
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Henriette LämmermannDepartment of Chemistry, University of Zurich, 8057, Zurich, Switzerland.
Jade NguyenInstitute of Chemical Sciences and Engineering, École Polytechnique Fédérale de Lausanne, 1015, Lausanne, Switzerland.
Juan F Tamez-FernándezDepartment of Chemistry, University of Zurich, 8057, Zurich, Switzerland.
Fabien KuttlerBiomolecular Screening Facility, École Polytechnique Fédérale de Lausanne, 1015, Lausanne, Switzerland.
Julien Bortoli ChapalayBiomolecular Screening Facility, École Polytechnique Fédérale de Lausanne, 1015, Lausanne, Switzerland.
Marc ChambonBiomolecular Screening Facility, École Polytechnique Fédérale de Lausanne, 1015, Lausanne, Switzerland.
Gerardo TurcattiBiomolecular Screening Facility, École Polytechnique Fédérale de Lausanne, 1015, Lausanne, Switzerland.
Pablo Rivera-FuentesDepartment of Chemistry, University of Zurich, 8057, Zurich, Switzerland.ORCID https://orcid.org/0000-0001-8558-2828

Funding

NCCR Chemical BiologySwiss National Science Foundation PCEGP2_186862
6 · The paper itself

Abstract

Stress response pathways rely on coordinated gene expression regulation. In the endoplasmic reticulum, the unfolded protein response plays an important role in maintaining the homeostasis of protein folding and processing. A chemigenetic reporter assay is developed that allows monitoring of BiP/GRP78 expression, a key regulator of the unfolded protein response. The system is based on the coexpression of the self-labeling protein HaloTag via an internal ribosome entry site. HaloTag allows for flexibility in labeling color as well as labeling timepoint. A two-color timestamping strategy is designed that provides improved readout sensitivity. The reporter system can be used in live-cell imaging as well as flow cytometry. It is applied in a high-content screening experiment in which a new promising activator of BiP/GRP78 expression was identified.

Indexed as

Genes, ReporterHeat-Shock ProteinsColorEndoplasmic Reticulum Chaperone BiPFlow CytometryHumansUnfolded Protein ResponseEndoplasmic Reticulum Chaperone BiPHeat-Shock ProteinsHSPA5 protein, humanBiP/GRP78HaloTaghigh‐content screeningreporter gene assays

Identifiers

PMID40939153
PMCPMC12498184

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.