Evidence map›Paper›PMID 40940329›Full record

ArticleScientific reports2025

Glycoprotein non-metastatic melanoma protein B is a potential biomarker for arthroplasty aseptic loosening.

Patrik Schadzek, Alexander Derksen, Wiebke Behrens, Maike Kosanke, Oliver Dittrich-Breiholz, Anika Hamm, Kirsten Elger, Yvonne Roger, Ines Yang, Meike Stiesch and 8 more

Abstract read
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In one paragraph

Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

18 authors.

Patrik Schadzek *Department of Orthopaedic Surgery, Biological Basics for Biohybrid Implants OE 8893, Hannover Medical School, Anna-von-Borries-Str. 1-7, Stadtfelddamm 34, 30625, Hannover, Germany.
Alexander Derksen *Department of Orthopaedic Surgery, Hannover Medical School and DIAKOVERE Annastift, Anna-von-Borries-Str. 1-7, 30625, Hannover, Germany.
Wiebke Behrens *Department of Prosthetic Dentistry and Biomedical Materials Science, Hannover Medical School, Carl-Neuberg-Str. 1, 30625, Hannover, Germany.
Maike KosankeResearch Core Unit Genomics, Hannover Medical School, Carl-Neuberg-Str. 1, 30625, Hannover, Germany.
Oliver Dittrich-BreiholzResearch Core Unit Genomics, Hannover Medical School, Carl-Neuberg-Str. 1, 30625, Hannover, Germany.
Anika HammDepartment of Orthopaedic Surgery, Biological Basics for Biohybrid Implants OE 8893, Hannover Medical School, Anna-von-Borries-Str. 1-7, Stadtfelddamm 34, 30625, Hannover, Germany.
Kirsten ElgerDepartment of Orthopaedic Surgery, Biological Basics for Biohybrid Implants OE 8893, Hannover Medical School, Anna-von-Borries-Str. 1-7, Stadtfelddamm 34, 30625, Hannover, Germany.
Yvonne RogerDepartment of Orthopaedic Surgery, Biological Basics for Biohybrid Implants OE 8893, Hannover Medical School, Anna-von-Borries-Str. 1-7, Stadtfelddamm 34, 30625, Hannover, Germany.
Ines YangDepartment of Prosthetic Dentistry and Biomedical Materials Science, Hannover Medical School, Carl-Neuberg-Str. 1, 30625, Hannover, Germany.
Meike StieschDepartment of Prosthetic Dentistry and Biomedical Materials Science, Hannover Medical School, Carl-Neuberg-Str. 1, 30625, Hannover, Germany.
Yvonne NollDepartment of Orthopaedic Surgery, Hannover Medical School and DIAKOVERE Annastift, Anna-von-Borries-Str. 1-7, 30625, Hannover, Germany.
Marco HaertléDepartment of Orthopaedic Surgery, Hannover Medical School and DIAKOVERE Annastift, Anna-von-Borries-Str. 1-7, 30625, Hannover, Germany.
Lars-René TueckingDepartment of Orthopaedic Surgery, Hannover Medical School and DIAKOVERE Annastift, Anna-von-Borries-Str. 1-7, 30625, Hannover, Germany.
Christina Stukenborg-ColsmanDepartment of Orthopaedic Surgery, Hannover Medical School and DIAKOVERE Annastift, Anna-von-Borries-Str. 1-7, 30625, Hannover, Germany.
Henning WindhagenDepartment of Orthopaedic Surgery, Hannover Medical School and DIAKOVERE Annastift, Anna-von-Borries-Str. 1-7, 30625, Hannover, Germany.
Doan Duy Hai TranDepartment of Nephrology and Rheumatology, University Medical Centre Göttingen, Robert-Koch-Str. 40, 37075, Göttingen, Germany.
Anette MelkDepartment of Paediatric Kidney, Liver and Metabolic Diseases, Hannover Medical School, Carl-Neuberg-Str. 1, 30625, Hannover, Germany.
Andrea HoffmannDepartment of Orthopaedic Surgery, Biological Basics for Biohybrid Implants OE 8893, Hannover Medical School, Anna-von-Borries-Str. 1-7, Stadtfelddamm 34, 30625, Hannover, Germany. hoffmann.andrea@mh-hannover.de.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Endoprosthesis loosening is the major cause of arthroplasty failure. Currently, radiography, histo-pathological classification of the periprosthetic membrane, and microbiological examination are used retrospectively for diagnosis. Prospective options for early diagnosis are not available. The study presented here aimed to identify (prospective) molecular biomarkers of implant loosening in tissue samples from patients. Four patient cohorts (primary and revision arthroplasty due to aseptic loosening of hip or knee) were defined. Aseptic loosening of implants was assessed by the standardised approach of the Knee Society Total Knee Arthroplasty Roentgenographic Evaluation and Scoring System. Synovial fluid, bone marrow, and blood were collected from 96 patients. Mesenchymal stromal cells (BM-MSCs) were isolated from the bone marrow. Bulk RNA-sequencing of 28 samples (including a fifth patient cohort with knee arthrofibrosis as aseptic cause for revision surgery) showed that Glycoprotein Non-Metastatic Melanoma Protein B mRNA (GPNMB) was significantly upregulated in BM-MSCs derived from revision patients compared to patients with primary implantations. Elevated GPNMB mRNA levels were confirmed with qRT-PCR. Synovial fluid plasma study by ELISA revealed increased levels of GPNMB protein in patients undergoing revision surgery compared to patients undergoing primary arthroplasty. GPNMB concentration in synovial fluid plasma, which can be obtained non-invasively, could be a potential biomarker for early detection of implant loosening, ahead of current diagnostic procedures.

Indexed as

Arthroplasty, Replacement, KneeMembrane GlycoproteinsProsthesis FailureAgedAged, 80 and overArthroplasty, Replacement, HipBiomarkersFemaleHumansKnee ProsthesisMaleMesenchymal Stem CellsMiddle AgedReoperationSynovial FluidBiomarkersGPNMB protein, humanMembrane GlycoproteinsBiomarkerDefect lengthEndoprosthesis looseningExtracellular domain sheddingOsteoactivinTranscriptome analysis

Identifiers

PMID40940329
PMCPMC12432123

What Socratic holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.