ArticlePolymers2025
The Role of Collagen Rheology in Human Keratinocyte Differentiation: Implications for Skin Substitute Development.
Article in Polymers, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
2 citing papers in PubMed.
- Electrospun collagen/hyaluronic acid dressing co-loaded with EGF and bFGF for enhanced diabetic wound healing.RSC advances · 2026Article
- Collagen Type I as a Biological Barrier Interface in Biomimetic Microfluidic Devices: Properties, Applications, and Challenges.Biomimetics (Basel, Switzerland) · 2026Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors.
Funding
Abstract
Type I collagen hydrogels are widely employed as scaffolds in tissue engineering due to their biocompatibility and ability to mimic the extracellular matrix (ECM). ECM viscoelasticity plays a critical role in regulating key cellular functions such as adhesion, proliferation, and differentiation. This study evaluates how collagen source and quality influence hydrogel architecture, mechanical properties, and keratinocyte behavior. Hydrogels were prepared at a concentration of 2.3 mg/mL using collagen from Advanced Biomatrix (AB, GLP grade) and Collagen Solutions (CS, GMP grade), and assessed for fibrillogenesis, rheological performance, and their ability to support stratified HaCaT keratinocyte cultures. AB-derived hydrogels exhibited higher porosity but lower mechanical resilience, characterized by a linear viscoelastic region (LVER) of 2.54%. In contrast, CS-derived hydrogels showed reduced porosity, denser fiber networks, and a higher LVER of 9.96%, indicating enhanced strain tolerance. HaCaT cells cultured on AB hydrogels showed diminished proliferation, metabolic activity, stratification, and expression of differentiation markers compared to those on CS hydrogels, which supported a more robust epidermal architecture. These findings highlight the critical role of collagen quality and mechanical characteristics on scaffold performance and epidermal tissue formation, emphasizing the need to optimize biomaterial properties for effective regenerative outcomes.
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What Socratic holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.