Evidence map›Paper›PMID 40943534›Full record

ReviewInternational journal of molecular sciences2025

Selecting Optimal Housekeeping Genes for RT-qPCR in Endometrial Cancer Studies: A Narrative Review.

Maciej Jóźwik, Iwona Sidorkiewicz, Joanna Wojtkiewicz, Stanisław Sulkowski, Andrzej Semczuk, Marcin Jóźwik

Abstract readReview
In one paragraph

Review in International journal of molecular sciences, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Maciej JóźwikDepartment of Gynecology and Gynecologic Oncology, Medical University of Białystok, 15-276 Białystok, Poland.
Iwona SidorkiewiczClinical Research Support Centre, Medical University of Białystok, 15-276 Białystok, Poland.ORCID 0000-0003-0254-3095
Joanna WojtkiewiczDepartment of Human Physiology and Pathophysiology, Faculty of Medicine, Collegium Medicum, University of Warmia and Mazury in Olsztyn, 10-082 Olsztyn, Poland.ORCID 0000-0003-0856-3644
Stanisław SulkowskiDepartment of General Pathomorphology, Medical University of Białystok, 15-269 Białystok, Poland.
Andrzej SemczukIInd Department of Gynecological Surgery and Gynecological Oncology, Lublin Medical University, 20-090 Lublin, Poland.ORCID 0000-0001-9344-9437
Marcin JóźwikDepartment of Gynecology and Obstetrics, Collegium Medicum, University of Warmia and Mazury in Olsztyn, 10-045 Olsztyn, Poland.ORCID 0000-0003-3401-2758

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Detailed analysis of gene expression by real time-quantitative polymerase chain reaction (RT-qPCR) has become a widespread method. To normalize the expression of target genes, this approach relies on constitutively expressed internal controls known as housekeeping genes (HKGs). Their proper selection is a critically important methodological step, since all the studied gene expression will be recalculated based on HKG expression. This concise review aims to discuss the selection of HKGs for endometrial cancer (EC) studies. We draw attention to the fact that the commonly used gene glyceraldehyde-3-phosphate dehydrogenase (

Indexed as

Endometrial NeoplasmsGenes, EssentialReal-Time Polymerase Chain ReactionBiomarkers, TumorFemaleGene Expression Regulation, NeoplasticGlyceraldehyde-3-Phosphate DehydrogenasesHumansBiomarkers, TumorGlyceraldehyde-3-Phosphate Dehydrogenasesendometrial cancerendometriumgeneshousekeepingpolymerase chain reaction

Identifiers

PMID40943534
PMCPMC12429465

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.