ArticleAnalytical chemistry2025
CaMK2rep: A Highly Sensitive Genetically Encoded Biosensor for Monitoring CaMKII Activity in Mammalian Cells.
Article in Analytical chemistry, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Accurately monitoring calcium/calmodulin-dependent protein kinase II (CaMKII) activity in cells remains a significant challenge due to the limited sensitivity and narrow dynamic range of existing genetically encoded sensors. Here, we introduce CaMK2rep, a novel phosphorylation-based biosensor that enables robust, specific, and high-sensitivity detection of CaMKII activity. CaMK2rep is designed with two tandem CaMKII consensus sites embedded within the native sequence context of synapsin, and its phosphorylation is detected via a phospho-specific antibody, allowing both biochemical and morphological analyses. We validated CaMK2rep in HeLa cells and cultured hippocampal neurons, demonstrating a near-linear response to CaMKII expression levels and to stimulation intensity, and no detectable cytotoxicity. To complement CaMK2rep measurements, we employed the live-cell CaMKAR (Reyes Gaido, O. E. et al.
Indexed as
Identifiers
What Socratic holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.