ArticleFrontiers in veterinary science2025
Development of an colloidal gold immunochromatography assay strip for the diagnosis of
Article in Frontiers in veterinary science, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
2 citing papers in PubMed.
- Advances and Challenges in the Diagnosis of Vector-Borne Protozoal Infections in Veterinary Medicine.Pathogens (Basel, Switzerland) · 2026Review
- Development of a colloidal gold immunochromatographic strip for the rapid detection of fowl adenovirus serotype 4 using prepared penton protein-specific monoclonal antibodies.Frontiers in veterinary science · 2026Article
Corrections and comments
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Authors and funding
11 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Introduction: Canine babesiosis, caused by Babesia canis, is a tick-borne hemolytic disease requiring rapid, reliable diagnostic tools to protect canine health in resource-limited settings. Methods: We developed a colloidal gold immunochromatographic assay (CGIA) strip using a recombinant BcMSA1-BcSA1 fusion protein, expressed in Escherichia coli with a yield of 2.5 mg/L, combining hydrophilic domains of merozoite surface antigen (BcMSA1) and secreted antigen (BcSA1). Seventy-two serum samples from veterinary clinics in Shanxi Province, China (ethical approval SXAU-2022-013), were tested against a commercial ELISA kit (Anigen Rapid B. canis Ab Test Kit), with infections confirmed by microscopy and PCR. Specificity was assessed using sera positive for Theileria spp., Toxoplasma gondii, Ancylostoma caninum, Eimeria canis, Canine distemper virus, and Canine parvovirus. Results: The CGIA strip showed no cross-reactivity, a detection limit of 1:8 for B. canis-positive sera, and retained efficacy after 18 months at room temperature. Sensitivity was 84%, specificity 93.6%, and Cohen's Kappa was 0.935 compared to ELISA. Discussion: This stable, user-friendly CGIA strip offers an efficient point-of-care solution for B. canis detection, overcoming limitations of traditional methods and supporting epidemiological and clinical applications.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.