Evidence map›Paper›PMID 40950097›Full record

ArticlebioRxiv : the preprint server for biology2025

The MLLT3 YEATS domain is a dual reader of histone marks (H3K9/18/27ac/cr) and ncRNA (7SK), linking epigenetic and RNA signaling to regulate hematopoiesis.

Adam Boulton, Ashish Kabra, Nicholas Achille, Emmalee R Adelman, Dimitrios G Anastasakis, Felipe Beckedorff, Pradeepkumar Cingaram, Ying Zhang, Benjamin Leach, Ramin Shiekhattar and 5 more

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

15 authors.

Adam BoultonDept. of Molecular Physiology and Biological Physics, University of Virginia, Charlottesville, VA 22903, United States.
Ashish KabraDept. of Molecular Physiology and Biological Physics, University of Virginia, Charlottesville, VA 22903, United States.
Nicholas AchilleDept. of Cancer Biology, Loyola University Chicago, Maywood, IL 60153, United States.
Emmalee R AdelmanDept. of Biochemistry, University of Miami Miller School of Medicine, Miami, FL 33136, United States.
Dimitrios G AnastasakisRNA Molecular Biology Laboratory, National Institute of Arthritis and Musculoskeletal and Skin Diseases, Intramural Research Program, National Institutes of Health, Bethesda, MD 20892, United States.
Felipe BeckedorffSylvester Comprehensive Cancer Center, University of Miami Miller School of Medicine, Miami, FL 33136, United States.
Pradeepkumar CingaramSylvester Comprehensive Cancer Center, University of Miami Miller School of Medicine, Miami, FL 33136, United States.
Ying ZhangDept. of Microbiology, Immunology and Cancer Biology, University of Virginia, Charlottesville, VA 22903, United States.
Benjamin LeachDept. of Molecular Physiology and Biological Physics, University of Virginia, Charlottesville, VA 22903, United States.
Ramin ShiekhattarSylvester Comprehensive Cancer Center, University of Miami Miller School of Medicine, Miami, FL 33136, United States.
Akihiko YokoyamaTsuruoka Metabolomics Laboratory, National Cancer Center, Tsuruoka, Yamagata, Japan.
Markus HafnerRNA Molecular Biology Laboratory, National Institute of Arthritis and Musculoskeletal and Skin Diseases, Intramural Research Program, National Institutes of Health, Bethesda, MD 20892, United States.ORCID 0000-0002-4336-6518
Maria FigueroaDept. of Biochemistry, University of Miami Miller School of Medicine, Miami, FL 33136, United States.
Nancy Zeleznik-LeDept. of Cancer Biology, Loyola University Chicago, Maywood, IL 60153, United States.
John BushwellerDept. of Molecular Physiology and Biological Physics, University of Virginia, Charlottesville, VA 22903, United States.ORCID 0000-0002-5386-1758

Funding

AF9(MLLT3) Function in Leukemia and Normal HematopoiesisR01CA233749 · NCI · UNIVERSITY OF VIRGINIA · PI BUSHWELLER, JOHN HACKETT, FIGUEROA, MARIA EUGENIA · 2019 to 2023
$3.2M
NCI NIH HHS R01 CA233749
6 · The paper itself

Abstract

The protein MLLT3 (AF9) is a critical regulator of hematopoiesis. The N-terminal YEATS domain of MLLT3 is an epigenetic reader that binds to acetylated as well as crotonylated lysine. Using PAR-CLIP, biochemical assays, and NMR based mapping of binding, we demonstrate that the YEATS domain of MLLT3 binds to a specific stem-loop region of the noncoding RNA 7SK. 7SK is a noncoding RNA with a well-documented function in transcriptional elongation. We developed point mutations in the YEATS domain that disrupt RNA binding while having no effect on binding of acetylated histone peptides to probe the specific role of RNA binding in MLLT3 function. Our results show loss of RNA binding by MLLT3 skews hematopoietic differentiation away from the myeloid lineage and toward the lymphoid lineage and has substantial effects on gene expression, confirming the essential nature of MLLT3-RNA binding for function.

Identifiers

PMID40950097
PMCPMC12424957

What Socratic holds

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LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.