ArticleFrontiers in cell and developmental biology2025
Integrating microarray data and single-cell RNA-seq reveals correlation between kit and nmyc in mouse spermatogonia stem cell population.
Article in Frontiers in cell and developmental biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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Who cites it
4 citing papers in PubMed.
- Identifying Mouse Undifferentiated To Differentiated Spermatogonia Stem Cells at the Single-Cell Level Using Machine Learning Approaches.Stem cell reviews and reports · 2026Article
- Machine learning, whole-transcriptome and integrative omics analysis reveals key regulatory networks governing human spermatogonial stem cells.Clinical and experimental medicine · 2026Article
- Analysis of microarray and single-cell RNA-seq finds gene co-expression, cell-cell communication, and tumor environment associated with cytoskeleton protein in epithelial-mesenchymal transition in ovarian cancer.Discover oncology · 2026Article
- Article
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3 authors.
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Abstract
Spermatogonial stem cells (SSCs) are essential for the continuous production of sperm and the maintenance of male fertility. Their selection, culture, and molecular characterization provide critical insights into spermatogenesis and potential therapeutic applications for male infertility. This study utilized CD49f-MACS and matrix selection techniques to isolate SSCs from mouse testicular samples. The molecular profile of the selected SSCs was analyzed through immunocytochemistry, gene ontology enrichment, weighted gene co-expression network analysis (WGCNA), and single-cell RNA sequencing (scRNA-seq). Additionally, protein-protein interaction (PPI) networks were constructed to identify key regulatory factors in SSC maintenance and differentiation. The selected SSCs exhibited a distinct molecular signature, with high expression of Dazl, Pou5f1 (Oct4), Gfra1, Nanog, and Kit. The Kit gene (c-kit) emerged as a crucial regulator of SSC differentiation, strongly associated with retinoic acid (RA)-mediated signaling pathways. Co-expression analysis revealed significant interactions between Kit, Nmyc, and other pluripotency-associated genes, highlighting its role in SSC development. Furthermore, single-cell RNA sequencing confirmed the dynamic expression of Kit during SSC differentiation and early meiosis initiation. Our findings underscore the pivotal role of Kit in spermatogenesis, reinforcing its potential as a therapeutic target for treating male infertility. The study also provides a comprehensive molecular framework for understanding SSC biology, with implications for regenerative medicine, fertility preservation, and
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