Evidence map›Paper›PMID 40958362›Full record

ArticleAustralian endodontic journal : the journal of the Australian Society of Endodontology Inc2026

Effect of Intracanal Medications on the Viability of Human Periodontal Ligament-Derived Mesenchymal Stem Cells.

Arian Braido, Walbert de Andrade Vieira, Bruno Cazotti Pereira, Karina Gonzales Silvério, Paulo Henrique Gabriel, Aline Cristine Gomes Matta, Emerson Alves Martins, Adriana de Jesus Soares

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Australian endodontic journal : the journal of the Australian Society of Endodontology Inc, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Effect of Intracanal Medications on the Viability of Human Periodontal Ligament-Derived Mesenchymal Stem Cells.Australian endodontic journal : the journal of the Australian Society of Endodontology Inc · 2026
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Arian BraidoDepartment of Restorative Dentistry, Endodontics Division, Piracicaba Dental School, Universidade Estadual de Campinas - UNICAMP, Piracicaba, SP, Brazil.ORCID https://orcid.org/0009-0007-8476-8402
Walbert de Andrade VieiraDepartment of Restorative Dentistry, Endodontics Division, Piracicaba Dental School, Universidade Estadual de Campinas - UNICAMP, Piracicaba, SP, Brazil.ORCID https://orcid.org/0000-0001-8872-2865
Bruno Cazotti PereiraDepartment of Restorative Dentistry, Endodontics Division, Piracicaba Dental School, Universidade Estadual de Campinas - UNICAMP, Piracicaba, SP, Brazil.ORCID https://orcid.org/0000-0001-5800-6366
Karina Gonzales SilvérioDepartment of Restorative Dentistry, Endodontics Division, Piracicaba Dental School, Universidade Estadual de Campinas - UNICAMP, Piracicaba, SP, Brazil.ORCID https://orcid.org/0000-0001-5879-9095
Paulo Henrique GabrielDepartment of Restorative Dentistry, Endodontics Division, Piracicaba Dental School, Universidade Estadual de Campinas - UNICAMP, Piracicaba, SP, Brazil.ORCID https://orcid.org/0000-0002-3075-9400
Aline Cristine Gomes MattaDepartment of Restorative Dentistry, Endodontics Division, Piracicaba Dental School, Universidade Estadual de Campinas - UNICAMP, Piracicaba, SP, Brazil.ORCID https://orcid.org/0000-0003-2789-5185
Emerson Alves MartinsDepartment of Restorative Sciences, University of Alabama at Birmingham, School of Dentistry, Birmingham, Alabama, USA.ORCID https://orcid.org/0009-0001-8762-0127
Adriana de Jesus SoaresDepartment of Restorative Dentistry, Endodontics Division, Piracicaba Dental School, Universidade Estadual de Campinas - UNICAMP, Piracicaba, SP, Brazil.ORCID https://orcid.org/0000-0002-8078-1606

Funding

Conselho Nacional de Desenvolvimento Científico e Tecnológico 303587/2022-9Coordenação de Aperfeiçoamento de Pessoal de Nível Superior 001Fundo de Apoio ao Ensino, à Pesquisa e Extensão, Universidade Estadual de Campinas
6 · The paper itself

Abstract

This in vitro study evaluated the effects of intracanal medications on the metabolic activity and adhesion of human periodontal ligament-derived mesenchymal stem cells. Tested groups included Control (without medication), Triple Antibiotic Paste (TAP), Double Antibiotic Paste (DAP), calcium hydroxide with saline, calcium hydroxide with 2% chlorhexidine gel and Bio-C Temp. After conditioning, roots were stored in phosphate-buffered saline at 37°C for 21 days. Medications were then removed using 17% EDTA and saline, and dentine samples were used as substrates for cell culture at 3, 5 and 7 days. Metabolic activity was assessed via MTT assay, and adhesion via scanning electron microscopy. None of the medications negatively affected cell viability or adhesion compared to control. TAP showed higher metabolic activity than calcium hydroxide with saline at Day 7. DAP induced earlier cell morphology changes. Bio-C Temp and chlorhexidine gel showed no significant differences at Day 7, confirming overall biocompatibility.

Indexed as

Anti-Bacterial AgentsMesenchymal Stem CellsPeriodontal LigamentRoot Canal IrrigantsCalcium HydroxideCell AdhesionCells, CulturedCell SurvivalChlorhexidineHumansIn Vitro TechniquesMicroscopy, Electron, ScanningAnti-Bacterial AgentsCalcium HydroxideChlorhexidineRoot Canal Irrigantscell survivalmesenchymal stem cellsregenerative endodonticsroot canal medicaments

Identifiers

PMID40958362
PMCPMC13051003

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.