Integrative transcriptomic and metabolomic analyses provide insights into the mechanism of autotoxicity of Pugionium cornutum (L.) Gaertn.

Kezhen Ning, Fenglan Zhang, Zhongren Yang, Xiaoyan Zhang, Dong Zhang, Xinyuan Qin, Xiumei Huang, Lizhen Hao

Abstract read

In one paragraph

Article in PloS one, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Kezhen NingCollege of Horticultural and Plant Protection, Inner Mongolia Agricultural University, Inner Mongolia Key Laboratory of Wild Peculiar Vegetable Germplasm Resource and Germplasm Enhancement, Huhhot, China.

Fenglan ZhangCollege of Horticultural and Plant Protection, Inner Mongolia Agricultural University, Inner Mongolia Key Laboratory of Wild Peculiar Vegetable Germplasm Resource and Germplasm Enhancement, Huhhot, China.ORCID https://orcid.org/0009-0009-5697-3112

Zhongren YangCollege of Horticultural and Plant Protection, Inner Mongolia Agricultural University, Inner Mongolia Key Laboratory of Wild Peculiar Vegetable Germplasm Resource and Germplasm Enhancement, Huhhot, China.

Xiaoyan ZhangCollege of Horticultural and Plant Protection, Inner Mongolia Agricultural University, Inner Mongolia Key Laboratory of Wild Peculiar Vegetable Germplasm Resource and Germplasm Enhancement, Huhhot, China.

Dong ZhangCollege of Horticultural and Plant Protection, Inner Mongolia Agricultural University, Inner Mongolia Key Laboratory of Wild Peculiar Vegetable Germplasm Resource and Germplasm Enhancement, Huhhot, China.

Xinyuan QinCollege of Horticultural and Plant Protection, Inner Mongolia Agricultural University, Inner Mongolia Key Laboratory of Wild Peculiar Vegetable Germplasm Resource and Germplasm Enhancement, Huhhot, China.

Xiumei HuangCollege of Horticultural and Plant Protection, Inner Mongolia Agricultural University, Inner Mongolia Key Laboratory of Wild Peculiar Vegetable Germplasm Resource and Germplasm Enhancement, Huhhot, China.

Lizhen HaoCollege of Horticultural and Plant Protection, Inner Mongolia Agricultural University, Inner Mongolia Key Laboratory of Wild Peculiar Vegetable Germplasm Resource and Germplasm Enhancement, Huhhot, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Pugionium cornutum (L.) Gaertn., a functional vegetable with dual health benefits, faces significant constraints in sustainable industrial development due to autotoxicity-induced continuous cropping obstacles. This study investigates the molecular regulatory mechanisms underlying the response of Pugionium cornutum (L.) Gaertn. to phthalic acid, a representative autotoxic substance, through integrated transcriptomic and metabolomic analyses under varying phthalic acid concentrations. The results revealed that phthalic acid stress significantly altered the abundance of 892 metabolites. Critical metabolites including MG(16:0/0:0/0:0)[rac] and 6-hydroxysphingosine were found to modulate differentially expressed genes (DEGs) involved in key pathways such as flavonoid biosynthesis, tropane/piperidine/pyridine alkaloid biosynthesis, glucosinolate metabolism, and ABC transporter activity. Comparative analysis demonstrated more pronounced molecular responses under high-concentration phthalic acid stress (10 mmol/L) compared to low-concentration treatment (0.1 m mmol/L), indicating intensified phytotoxic effects at elevated autotoxin levels. These findings provide novel insights into the autotoxicity response mechanisms of Pugionium cornutum (L.) Gaertn.and establish a theoretical foundation for developing sustainable cultivation strategies for this species.

Indexed as

MetabolomeMetabolomicsTranscriptomeGene Expression ProfilingGene Expression Regulation, Plant

Identifiers

PMID40961100

DOI10.1371/journal.pone.0331858

PMCPMC12443292

What Socratic holds

Textmetadata

LicenceCC BY

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.