Evidence map›Paper›PMID 40964049›Full record

ArticleFrontiers in cellular and infection microbiology2025

Single-nucleus RNA sequencing reveals HBV-driven metabolic reprogramming and TIMP1-mediated fibrosis in human-liver-chimeric mice.

Xiaonan Ren, Cong Wang, Boyin Qin, Hua Yang, Min Wu, Zhanqing Zhang, Wei Lu, Chao Wang, Yabin Liu, Xiaonan Zhang and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Frontiers in cellular and infection microbiology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 synthesis or guideline pooled it.

  1. Single-cell RNA sequencing offers novel perspectives in viral infection research.Frontiers in cellular and infection microbiology · 2026
    Pooled it
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Xiaonan Ren *Department of Laboratory Animal Science, Shanghai Public Health Clinical Center, Fudan University, Shanghai, China.
Cong Wang *Department of Basic Scientific Research, Shanghai Public Health Clinical Center, Fudan University, Shanghai, China.
Boyin QinDepartment of Laboratory Animal Science, Shanghai Public Health Clinical Center, Fudan University, Shanghai, China.
Hua YangDepartment of Laboratory Animal Science, Shanghai Public Health Clinical Center, Fudan University, Shanghai, China.
Min WuDepartment of Basic Scientific Research, Shanghai Public Health Clinical Center, Fudan University, Shanghai, China.
Zhanqing ZhangDepartment of Hepatology, Shanghai Public Health Clinical Center, Fudan University, Shanghai, China.
Wei LuDepartment of Hepatology, Shanghai Public Health Clinical Center, Fudan University, Shanghai, China.
Chao WangDepartment of Laboratory Animal Science, Shanghai Public Health Clinical Center, Fudan University, Shanghai, China.
Yabin LiuDepartment of Basic Scientific Research, Shanghai Public Health Clinical Center, Fudan University, Shanghai, China.
Xiaonan ZhangDepartment of Basic Scientific Research, Shanghai Public Health Clinical Center, Fudan University, Shanghai, China.
Xiaohui ZhouDepartment of Laboratory Animal Science, Shanghai Public Health Clinical Center, Fudan University, Shanghai, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Introduction: Hepatitis B virus (HBV) infection remains a leading cause of chronic liver disease, cirrhosis, and hepatocellular carcinoma worldwide. Despite advances in antiviral therapies, the mechanisms underlying HBV-induced metabolic reprogramming and liver fibrosis remain poorly understood. Methods: We employed single-nucleus RNA sequencing (snRNA-seq) which is particularly suitable for hepatocytic sequencing to dissect the transcriptional landscape of HBV-infected and uninfected hepatocytes in humanized URG mice (Hu-URG). Results and Discussion: Chronic HBV infection was successfully established in Hu-URG mice, with progressive increases in serum HBV DNA, HBsAg, and HBeAg levels. snRNA-seq revealed distinct human hepatocyte clusters (clusters 9, 16, 23) characterizing elevated expression of metabolic genes (

Indexed as

Hepatitis B, ChronicHepatitis B virusLiver CirrhosisTissue Inhibitor of Metalloproteinase-1AnimalsDisease Models, AnimalDNA, ViralHepatitis B Surface AntigensHepatocytesHumansLiverMetabolic ReprogrammingMiceSequence Analysis, RNADNA, ViralHepatitis B Surface AntigensTIMP1 protein, humanTissue Inhibitor of Metalloproteinase-1ALBHBVliver humanized micesnRNA-seqTIMP1URG

Identifiers

PMID40964049
PMCPMC12436295

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.